A comparative analysis of differentially expressed genes in rostral and caudal regions after spinal cord injury in rats  

作　　者：Xue-Min Cao Sheng-Long Li Yu-Qi Cao Ye-Hua Lv Ya-Xian Wang Bin Yu Chun Yao 

机构地区：[1]Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education,NMPA Key Laboratory for Research and Evaluation of Tissue Engineering Technology Products,Co-innovation Center of Neuroregeneration,Nantong University,Nantong,Jiangsu Province,China

出　　处：《Neural Regeneration Research》2022年第10期2267-2271,共5页中国神经再生研究（英文版）

基　　金：supported by Postgraduate Research&Practice Innovation Program of Jiangsu Province,No.KYCX-2065(to XMC).

摘　　要：The initial mechanical damage of a spinal cord injury(SCI)triggers a progressive secondary injury cascade,which is a complicated process integrating multiple systems and cells.It is crucial to explore the molecular and biological process alterations that occur after SCI for therapy development.The differences between the rostral and caudal regions around an SCI lesion have received little attention.Here,we analyzed the differentially expressed genes between rostral and caudal sites after injury to determine the biological processes in these two segments after SCI.We identified a set of differentially expressed genes,including Col3a1,Col1a1,Dcn,Fn1,Kcnk3,and Nrg1,between rostral and caudal regions at different time points following SCI.Functional enrichment analysis indicated that these genes were involved in response to mechanical stimulus,blood vessel development,and brain development.We then chose Col3a1,Col1a1,Dcn,Fn1,Kcnk3,and Nrg1 for quantitative real-time PCR and Fn1 for immunostaining validation.Our results indicate alterations in different biological events enriched in the rostral and caudal lesion areas,providing new insights into the pathology of SCI.

关 键 词：biological process CAUDAL differentially expressed genes Gene Ontology HEMISECTION IMMUNOSTAINING Rattus norvegicus RNA-sequencing rostral spinal cord injury 

分 类 号：R-332[医药卫生] R651.2