Magnetic labeling of primary murine monocytes using very small superparamagnetic iron oxide nanoparticles  

作　　者：Martin Pohland Christoph Pohland Jürgen Kiwit Jana Glumm 

机构地区：[1]Institute of Cell Biology and Neurobiology,Center for Anatomy,Charité-Universitätsmedizin Berlin,Berlin,Germany [2]Department of Neurosurgery,Helios Klinikum Berlin Buch,Berlin,Germany

出　　处：《Neural Regeneration Research》2022年第10期2311-2315,共5页中国神经再生研究（英文版）

基　　金：supported by Deutsche Forschungsgemeinschaft(DFG)grant Klinische Forschergruppe(KFO)213(to JG).

摘　　要：Due to their very small size,nanoparticles can interact with all cells in the central nervous system.One of the most promising nanoparticle subgroups are very small superparamagnetic iron oxide nanoparticles(VSOP)that are citrate coated for electrostatic stabilization.To determine their influence on murine blood-derived monocytes,which easily enter the injured central nervous system,we applied VSOP and carboxydextran-coated superparamagnetic iron oxide nanoparticles(Resovist).We assessed their impact on the viability,cytokine,and chemokine secretion,as well as iron uptake of murine blood-derived monocytes.We found that(1)the monocytes accumulated VSOP and Resovist,(2)this uptake seemed to be nanoparticle-and time-dependent,(3)the decrease of monocytes viability was treatment-related,(4)VSOP and Resovist incubation did not alter cytokine homeostasis,and(5)overall a 6-hour treatment with 0.75 mM VSOP-R1 was probably sufficient to effectively label monocytes for future experiments.Since homeostasis is not altered,it is safe to label blood-derived monocles with VSOP.VSOP labeled monocytes can be used to study injured central nervous system sites further,for example with drug-carrying VSOP.

关 键 词：CD11b cytokine FERUCARBOTRAN Mac1 MPS MRI Resovist superparamagnetic iron oxide nanoparticles(SPIO) very small superparamagnetic iron oxide nanoparticles(VSOP) viability 

分 类 号：R741[医药卫生—神经病学与精神病学]