健康成年人舌苔表象与舌苔微生态的关系  

Relationship between tongue coating appearance and tongue coating microbiota in healthy adults

作　　者：肖池 何彩红[2] 王安鸽 谭中菊 赵新秀[2] 廖俏云 李金优 归崎峰[2] XIAO Chi;HE Caihong;WANG Ange;TAN Zhongju;ZHANO Xinxiu;LIAO Qiaoyun;LI Jinyou;GUI Qifeng(School of Basic Medical Sciences and Forensic Medicine,Hangzhou Medical College,Hangzhou 310003,China;不详)

机构地区：[1]杭州医学院基础医学与法医学院,杭州310003 [2]浙江大学医学院附属第一医院老年医学科

出　　处：《山东医药》2022年第1期30-33,I0001,共5页Shandong Medical Journal

基　　金：国家重点研发项目(2018YFC2000500);浙江省中医药科学研究基金计划(2020ZB148)。

摘　　要：目的探讨健康成年人舌苔表象与舌苔微生态的关系。方法选择健康成年志愿者79例,根据舌苔质地分为腻苔组43例、薄苔组36例,收集舌苔样本,提取基因组DNA,经PCR扩增纯化和文库构建后测序并进行生物信息学分析。结果79例健康成年志愿者舌苔标本中共检出2099个操作分类单位(OTUs)。其中,腻苔组和薄苔组共享的OTUs共1233个,腻苔组和薄苔组独有的OTUs分别为458、408个,两组OTUs比较差异无统计学意义(P>0.05)。α多样性分析发现,两组Chao1、ACE、Shannon、Simpson、Goods coverage、Observed species指数比较差异均无统计学意义(P均>0.05)。基于Weighted UniFrac距离进行的主坐标分析未发现两组菌群结构存在明显差异,主成分分析同样未发现两组菌群结构存在明显差异。线性判别分析(LDA)值分布柱状图展示了LDA>4的差异物种,其中腻苔组放线菌纲、乳杆菌目、微球菌目和链球菌科是差异物种,而薄苔组紫单胞菌科是差异物种。在进化分支图上同样显示了两组差异物种,并且在由门至属的分类级别上揭示了差异物种的所属关系。结论健康成年人中不同舌苔表象具有不同的舌苔微生态特征,其中放线菌纲、乳杆菌目、微球菌目和链球菌科是腻苔的差异物种,紫单胞菌科是薄苔的差异物种,这些菌群差异可能是不同舌苔类型形成的微生态基础。Objective To explore the correlation between different tongue coating appearances and tongue coating microbiota in healthy adults.Methods Seventy-nine healthy adults were enrolled and divided into the greasy tongue coating group(43 cases)and thin tongue coating group(36 cases)according to different tongue coatings.The tongue coating surface samples were collected and were used for genomic DNA extracting;bioinformatics analysis was performed after PCR amplification,purification,and library construction.Results A total of 2099 operational taxonomic units(OTUs)were detected in 79 healthy adult tongue coating samples,of which,1233 OTUs were common between the two groups,whereas 458 OTUs and 408 OTUs were unique to the greasy tongue coating group and the thin tongue coating group,respectively,and there was no significant difference in OTUs between the two groups(all P>0.05).The α diversity analysis showed that Chao1,ACE,Shannon,Simpson,Goods coverage,and Observed species indexes were not significantly different in the two groups(all P>0.05).Principal Coordinate Analysis based on Weighted Unifrac distance showed no significant difference between the two groups.Principal Component Analysis also confirmed that there was no significant difference between the two groups.The LDA value distribution histogram showed the significantly different species whose LDA score was greater than 4,the Actinobacteria,Lactobacillales,Micrococcales and Streptococcaceae were the significantly different species of greasy tongue coating group,and the Porphyromonadaceae was the significantly different species of the thin tongue coating group.The cladogram diagram showed the microbial species with significant differences between the two groups,and represented the classification level from phylum to species.Conclusion In healthy adults,different tongue coating appearances show different tongue microbiota characteristics;Actinobacteria,Lactobacillales,Micrococcales,and Streptococcaceae are the significantly different species of greasy tongue coat

关 键 词：舌苔 舌苔菌群 舌苔微生态 高通量测序 健康成年人 

分 类 号：R78[医药卫生—口腔医学]