米曲霉CPA毒素的5-氟色氨酸分子探针研究  

5-F-Trp as A Molecular Probe for CPA Toxins in Aspergillus oryzae

作　　者：江峥 胡江春[1] 王楠[1,2] JIANG Zheng;HU Jiangchun;WANG Nan(Institute of Applied Ecology,Chinese Academy of Sciences,Shenyang 110016,China;Ocean College,Zhejiang University,Zhoushan 316021,China;Graduate School of Chinese Academy of Sciences,Beijing 100049,China)

机构地区：[1]中国科学院沈阳应用生态研究所,辽宁沈阳110016 [2]浙江大学海洋学院,浙江舟山316021 [3]中国科学院大学研究生院,北京100049

出　　处：《食品与生物技术学报》2022年第2期46-50,共5页Journal of Food Science and Biotechnology

基　　金：国家自然科学基金项目(31471814);浙江大学学科与人才队伍建设专项项目(129000-541320/001/002)。

摘　　要：米曲霉中往往含有完整的环匹阿尼酸(cyclopiazonic acid,CPA)毒素生物合成基因簇。鉴于CPAs对食品安全的潜在威胁和其不同的存在形式,依据CPA的生物合成机制推测可将外源氟代前体探针化合物嵌入CPA生物合成途径,从而形成氟代物用于靶向检测。结果显示,米曲霉CPA合成途径可以接受5-F-L-Trp为底物并经CpaS酶合成5-F-cAATrp。由于该化合物并不是其下游催化酶CpaD的合适底物,因而无法启动下游后修饰步骤,造成了5-F-cAATrp在菌株中富集。利用CPA生物合成途径对代谢产物的富集效应实现对CPA毒素更加方便和特异性的检测,这对于建立新型CPA毒素检测方法,保障食品安全具有积极意义。Aspergillus oryzae often contains intact biosynthetic gene cluster for producing cyclopiazonic acid(CPA) toxins. As a result, CPAs has been recognized as a potential threat to food safety. Since CPAs may present in different forms of derivatives, we designed a strategy for incorporating exogenous fluorinated precursor into the CPA biosynthesis pathway. The results showed that after 5-F-L-Trp was used as the substrate and a molecular probe, it was accepted as the substrate of the first biosynthetic enzyme CpaS, producing 5-F-cAATrp, the very first biosynthetic intermediate. Meanwhile,5-F-cAATrp seemed an unsuitable substrate for the next catalytic enzyme CpaD, resulting abolishment of downstream tailoring steps and significant accumulation of this compound. The enrichment effect of CPA metabolites by the molecular probe therefore provides a new opportunity for more convenient and specific detection of CPA toxins targeting only one analyte.These findings are of significance for develop new method for detect and control the CPA toxins in Aspergillus-associated food products.

关 键 词：米曲霉 环匹阿尼酸 5-氟色氨酸 分子探针 

分 类 号：Q93[生物学—微生物学]