MiR-24对肺癌A549细胞增殖和迁移的影响  

Effect of miR-24 on the proliferation and migration of lung cancer A549 cells

作　　者：杜娟[1] 严馨[1] 邱丽[1] 易静叶 阳甜[1] DU Juan;YAN Xin;QIU Li;YI Jing-ye;YANG Tian(Department of Respiratory and Critical Care Medicine,the First Affiliated Hospital of Xi'an Jiaotong University,Xi'an 710061,Shaanxi,China)

机构地区：[1]西安交通大学第一附属医院呼吸与危重症医学科,陕西西安710061

出　　处：《川北医学院学报》2022年第2期143-146,共4页Journal of North Sichuan Medical College

基　　金：陕西省重点研发项目(2019KW-034)。

摘　　要：目的:分析MiR-24对肺癌A549细胞增殖和迁移的影响,并探讨其作用机制。方法:人肺癌A549细胞分为观察组(MiR-24拟似物)、抑制组(MiR-24抑制物)和对照组,培养0、12、24和48 h;MTT法、划痕实验和Transwell法检测MiR-24对人肺癌A549细胞增殖和迁移能力,qPCR和Western blot检测MiR-24对肺癌A549相关因子(MMP-2和MMP-9)mRNA和蛋白表达的影响。结果:0 h时,观察组和抑制组的增殖活力与对照组比较,差异无统计学意义(P>0.05);在12、24和48 h,观察组的细胞增殖活力低于抑制组(P<0.05);观察组的迁移细胞数、侵袭细胞数和愈合率均低于对照组和抑制组(P<0.05),且对照组低于抑制组(P<0.05);观察组的MMP-2和MMP-9蛋白表达和mRNA表达水平均低于对照组和抑制组(P<0.05),且对照组低于抑制组(P<0.05)。结论:MiR-24可有效抑制肺癌A549细胞的增殖、迁移和侵袭,且其作用机制可能与下调MMP-2和MMP-9的表达有关。Objective:To analyze the effects of miR-24 on proliferation and migration of lung cancer A549 cells and explore its mechanism.Methods:Human lung cancer A549 cells were divided into observation group(miR-24 agglomerate),inhibitory group(miR-24 inhibitory)and control group,cultured 0,12,24 and 48 h.MTT method,scratch experiment and Transwell method MiR-24 was detected by human lung cancer A549 cell proliferation and migration,qPCR and Western blot were used to detect the effect of MiR-24 on the mRNA and protein expression of A549 related factor(MMP-2 and MMP-9)in lung cancer.Results:At 0 h,there was no significant difference in proliferation activity between the observation group,the inhibitory group and the control group(P>0.05),and the cell proliferation activity of the observation group was lower than that of the inhibitory group at 12,24 and 48 h(P<0.05).The number of migration cells,the number of invasion cells and the healing rate of the observation group were lower than those of the control group and inhibitory group(P<0.05),and the control group was lower than the inhibitory group(P<0.05).The MMP-2 and MMP-9 protein expression and mRNA expression of the observation group was lower than those of the control group and inhibitory group(P<0.05),and the control group was lower than the inhibitory group(P<0.05).Conclusion:MIR-24 can effectively inhibit proliferation,migration and invasion of lung cancer A549 cells,and its mechanism may be related to down regulating the expression of MMP-2 and MMP-9.

关 键 词：MiR-24 肺癌细胞 细胞增殖 细胞迁移 基质金属蛋白酶 

分 类 号：R73-36[医药卫生—肿瘤] R734.2[医药卫生—临床医学]