Hsp90α联合TPA、CRP检测在恶性肿瘤临床诊断中的意义  

Significance of combined detectin of Hsp90αwith TPA and CRP in the clinical diagnosis of malignant tumors

作　　者：杜琴[1,2,3] 王强 蔡艳娟[1] 杜娟 张茂鑫[1] 郭晓兰[1,2,3] DU Qin;WANG Qiang;CAI Yan-juan;DU Juan;ZHANG Mao-xin;GUO Xiao-lan(Department of Laboratory Medicine,Affiliated Hospital,North Sichuan Medical College,Nanchong 637000,Sichuan,China;College of Laboratory Medicine,North Sichuan Medical College,Nanchong 637000,Sichuan,China;Center for Translational Medicine,North Sichuan Medical College,Nanchong 637000,Sichuan,China)

机构地区：[1]川北医学院附属医院检验科,四川南充637000 [2]川北医学院医学检验系,四川南充637000 [3]川北医学院转化医学研究中心,四川南充637000

出　　处：《川北医学院学报》2022年第2期190-193,共4页Journal of North Sichuan Medical College

基　　金：四川省南充市科技局市校合作基础研究项目(19SXH0244);南充市科技局2020年市级应用技术研究与开发资金项目(20YFZJ0095)。

摘　　要：目的:探讨热休克蛋白90α(Hsp90α)联合组织多肽抗原(TPA)、C反应蛋白(CRP)检测在恶性肿瘤临床诊断中的意义。方法:选取74例术后病理明确诊断为肺部和消化道恶性肿瘤的初治患者为肿瘤组,另选取48例同期肺部和消化道炎症性疾病患者(炎症组)及49名健康体检者(非炎症组)为对照组。比较三组对象外周血Hsp90α、TPA和CRP的表达水平;ROC曲线分析Hsp90α、TPA和CRP检测对恶性肿瘤的诊断效能。结果:与非炎症患者相比,恶性肿瘤患者的Hsp90α、TPA表达水平升高,差异有统计学意义(P<0.05)。ROC曲线分析显示,Hsp90α、TPA和CRP诊断恶性肿瘤的曲线下面积(AUC)分别为0.593、0.587、0.321;Hsp90α诊断的灵敏度最高可达74.3%,但特异性稍显不足,仅为40.2%。Hsp90α与CRP联合检测,AUC可提高到0.784,灵敏度和特异性增加,分别为81.1%和61.9%;增加检测TPA,并不能提高恶性肿瘤诊断的敏感度和检出率。Hsp90α/CRP与TPA/CRP诊断恶性肿瘤的AUC分别为0.765、0.742,灵敏度分别为82.4%和89.2%,但特异度稍降低,分别为58.8%和52.6%。结论:Hsp90α在恶性肿瘤的筛查方面较TPA更具优势,但特异性稍显不足,其联合CRP检测或进行Hsp90α与CRP比值计算,可有效提高诊断的敏感性和特异性,提升对恶性肿瘤的诊断效能。Objective:To explore the significance of Hsp90αcombined with TPA and CRP in the clinical diagnosis of malignant tumors.Methods:74 newly treated patients with lung and digestive tract malignant tumors confirmed by postoperative pathology were selected as the tumor group,and 48 patients with lung and digestive tract inflammatory diseases(inflammatory group)and 49 healthy people(non-inflammatory group)were selected as the control group.The expression levels of Hsp90α,TPA and CRP in peripheral blood were compared.ROC curve was used to analyze the diagnostic efficacy of Hsp90α,TPA and CRP for malignant tumors.Results:The expression levels of Hsp90αand TPA in tumor group was significantly higher than those in non-inflammation group,the differences were statistically significant(P<0.05).ROC curve analysis showed that the AUC were:Hsp90α(0.593)>TPA(0.587)>CRP(0.321),the sensitivity of malignant tumor in Hsp90αwas 74.3%,but the specificity was slightly insufficient,only 40.2%.The combined detection of Hsp90αwith CRP,the AUC could be increased to 0.784,the sensitivity and specificity were increased to 81.1%and 61.9%,respectively.Increasing the detection of TPA does not improve the sensitivity and detection rate of malignant tumors.After calculating the ratio of Hsp90α,TPA with CRP,the AUC was:Hsp90α/CRP(0.765),TPA/CRP(0.742),the sensitivity were 82.4%and 89.2%,but the specificity was slightly reduced,58.8%and 52.6%.Conclusion:Hsp90αhas high efficacy than TPA in the screening of malignant tumors,but its specificity is slightly insufficient.The combined detection of Hsp90αand CRP or the calculation of the ratio of Hsp90αto CRP can effectively improve its diagnostic sensitivity and specificity,so as to improve the diagnostic efficiency of malignant tumors.

关 键 词：热休克蛋白90Α 组织多肽抗原 C反应蛋白 联合检测 恶性肿瘤 诊断效能 

分 类 号：R735[医药卫生—肿瘤]