LncRNA-ROR在缺氧诱导胃癌侵袭转移中的作用  

Effect of LncRNA-ROR on the Hypoxia-Induced Gastric Cancer Metastasis and Invasion

作　　者：朱长明 孙高 陈功华 龚于刚 夏杰 ZHU Changming;SUN Gao;CHEN Gonghua;GONG Yugang;XIA Jie(Department of Gastrointestinal Surgery, Zhangjiajie People's Hospital Affiliated to Jishou University, Zhangjiajie 427000, Hunan China;School of Medical, Jishou University, Jishou 416000, Hunan China)

机构地区：[1]吉首大学附属张家界市人民医院胃肠外科,湖南张家界427000 [2]吉首大学医学院,湖南吉首416000

出　　处：《吉首大学学报（自然科学版）》2021年第6期48-53,共6页Journal of Jishou University(Natural Sciences Edition)

摘　　要：目的探讨LncRNA-ROR在缺氧诱导胃癌侵袭转移中的作用.方法采用RT-PCR法检测胃癌组织标本和胃癌细胞株中ROR mRNA的表达差异;通过基因重组方法构建ROR mRNA过表达载体,并转染SGC-7901和MGC-803胃癌细胞,再使用RT-PCR法检测过表达转载效果;通过Transwell试验研究缺氧环境下LV5-ROR对胃癌细胞SGC-7901和MGC-803侵袭及转移能力的影响.结果ROR mRNA在胃癌组织中的表达低于癌旁组织,同时,在缺氧诱导下胃癌细胞株中的ROR mRNA表达明显下降;缺氧能够促进SGC-7901和MGC-803细胞的侵袭及转移,而过表达ROR mRNA能够明显抑制缺氧条件下SGC-7901及MGC-803细胞的侵袭及转移.结论LncRNA-ROR能抑制胃癌细胞的侵袭及转移,为胃癌靶向治疗提供理论依据.Objective To investigate the effect of LncRNA ROR on metastasis and invasion in the hypoxia-induced invasion and metastasis in GC.Methods Using quantitative real-time polymerase chain reaction(RT-PCR)to detect the expression of ROR ingastric cancer tissues and cell lines.Employing the over-expression technique were used to observe the effect of overexpression of ROR on human gastric cancer cell metastasis and invasion,the transfection efficiency was verified by RT-PCR in SGC-7901 and MGC-803.Results ROR was significantly down-regulated in gastric cancer tissues samples compared with adjacent normal gastric tissues,and also down-regulated inthe hypoxic—induced GC cells.Hypoxia significantly increased the migration and invasion potential of SGC-7901 and MGC-803,After over-expression of ROR the hypoxia-induced migration and invasion of SGC7901 and MGC-803 cells were dramatically diminished.Conclusion LncRNA-ROR expression is decreased in hypoxic GC and suppressed hypoxia-induced GC metastasis and invasion,which could be a new biomarkers,a potential therapeutic target of gastric cancer.

关 键 词：胃癌 ROR 长链非编码RNA 侵袭转移 

分 类 号：R735.2[医药卫生—肿瘤]