水稻抗白叶枯病基因全基因组关联分析  

Genome-wide association study on rice bacterial blight resistance genes

作　　者：刘茁 彭莎莎 陈建芝 周亚林 李成刚 王丹[1] LIU Zhuo;PENG Shasha;CHEN Jianzhi;ZHOU Yalin;LI Chenggang;WANG Dan(College of Agronomy,Hunan Agricultural University,Changsha,Hunan 410128,China;State Key Laboratory of Hybrid Rice,Hunan Academy of Agricultural Sciences,Changsha,Hunan 410125,China;Bureau of Agriculture and Rural Affairs of Hengyang Municipality,Hengyang,Hunan 421001,China;Agricultural and rural Bureau of Mayang Miao Autonomous County,Huaihua,Hunan 419400,China)

机构地区：[1]湖南农业大学农学院,湖南长沙410128 [2]杂交水稻国家重点实验室,湖南长沙410125 [3]衡阳市农业农村局,湖南衡阳421001 [4]麻阳苗族自治县农业农村局,湖南怀化419400

出　　处：《湖南农业大学学报（自然科学版）》2022年第1期46-53,共8页Journal of Hunan Agricultural University(Natural Sciences)

基　　金：湖南省农业科技创新资金项目(2021CX35)。

摘　　要：对水稻多样性群体(RDP–I)中的216份种质接种白叶枯生理小种P2并进行抗性鉴定,发现温带粳稻亚群平均抗性水平最高,其平均病斑长度最短;奥斯稻亚群平均抗性水平最低,平均病斑长度最长。通过全基因组关联作图鉴定了分布在水稻第1、2、4、6、7、8、9、10、11、12号染色体上的59个QTL,这些位点包含5个已知的抗白叶枯病基因。从较高阈值SNP位点以及附近2 Mb区段进行候选基因的预测,筛选出40个抗白叶枯病相关基因,并最终鉴定出16个抗性较好的水稻种质资源。Resistance of rice diversity panel 1(RDP-I)including 216 varieties against bacterial blight was identified by inoculating the varieties with Xoo P2.Temperate japonica rice showed the highest average resistant level,with the shortest average length of disease spot;aus rice subpopulation showed the lowest average resistance,with the longest average length of disease spot.Genome-wide association study(GWAS)was used to analysis the resistance against the XooP2 of the Rice Diversity Panel 1(RDP-I).Disease evaluation of the RDP-I indicated that the TEJ subpopulation conferred a higher level of resistance to P2 than other subpopulations.Genome-wide association study(GWAS)showed that 59 QTLs were identified that distributing on chromosomes1,2,4,6,7,8,9,10,11 and 12 respectively,five cloned rice bacterial blightresistance genes were also located in the regions.From the higher p value SNP site and the 2 Mb segment near the site,we performed the prediction and screened 40 anti-bacterial blight associated candidate genes,and 16 rice varieties with high resistance against bacterial blight were identified.

关 键 词：水稻 白叶枯病抗性 全基因组关联分析 数量性状位点 

分 类 号：Q75[生物学—分子生物学]