柴油污染土壤烷烃降解菌的筛选及降解特性研究  

Screening and degradation characteristics of bacteria for degrading alkane in diesel contaminated soil

作　　者：王兴龙 颜家保 胡杰 鲍彦舟 WANG Xing-long;YAN Jia-bao;HU Jie;BAO Yan-zhou(Hubei Province Key Laboratory of Coal Conversion and New Carbon Materials,School of Chemistry and Chemical Engineering,Wuhan University of Science and Technology,Wuhan 430081,China)

机构地区：[1]武汉科技大学化学与化工学院,煤转化与新型炭材料湖北省重点实验室,湖北武汉430081

出　　处：《现代化工》2022年第2期163-166,共4页Modern Chemical Industry

基　　金：湖北省科技创新专项重大项目(2017ACA179)。

摘　　要：从某炼油厂柴油罐区污染土壤中分离筛选出优势柴油降解菌L12,并经过形态学观察、生理生化试验以及16S rDNA序列分析,鉴定为不动杆菌属(Acinetobacter.sp)。采用摇瓶实验研究了菌株L12对正十六烷的适宜降解条件,并考察了该菌对柴油的降解能力。结果表明,菌株L12最佳的培养初始pH为7、接种量为5%、摇床转速为150 r/min、培养温度为30℃,菌株可耐受正十六烷质量浓度高达5000 mg/L。最优条件下培养7 d,该菌株对正十六烷的降解率高达90.33%,能将质量浓度为3000 mg/L的柴油几乎完全降解,表明该菌具有良好的用于生物修复柴油污染土壤的潜力。L12,a dominant diesel-degrading bacterium,is isolated and screened out from the contaminated soil in the diesel tank area of a refinery.After morphological observation,physiological tests,biochemical tests and 16S rDNA sequence analysis,it is identified as Acinetobacter.sp.The shake flask experiment is utilized to study the suitable degradation conditions of L12 strain to n-hexadecane,and the degradation ability of L12 strain to diesel is investigated.The optimal cultural conditions for L12 strain are obtained as follows:the initial pH is 7,the inoculum amount is 5%,the shaking speed is 150 r·min-1,and the culture temperature is at 30℃.The strain can tolerate the concentration of nhexadecane up to 5000 mg·L^(-1).If L12 strain has been cultivated for 7 days under the optimal conditions,the strain's degradation rate to n-hexadecane is as high as 90.33%,it can almost completely degrade diesel fuel with a concentration of 3000 mg·L^(-1)within 7 days,indicating that the bacteria has a good potential for bioremediation of diesel polluted soil.

关 键 词：正十六烷 优势菌 降解特性 柴油 

分 类 号：TH3[机械工程—机械制造及自动化]