GKK1032B from endophytic Penicillium citrinum induces the apoptosis of human osteosarcoma MG63 cells through caspase pathway activation  

作　　者：LIU Na SONG Mei-Na ZHANG Qian-Qian WU Cong ZHU Kong-Kai SUN Yu-Lin LI Meng-Ru YANG Feng-Ying FENG Run-Liang ZHANG Yu-Ying ZHANG Hua 

机构地区：[1]School of Biological Science and Technology,University of Jinan,Jinan 250022,China

出　　处：《Chinese Journal of Natural Medicines》2022年第1期67-73,共7页中国天然药物（英文版）

基　　金：the National Natural Science Foundation of China(Nos.82073728 and 31501104);the Natural Science Foundation of Shandong Province(Nos.ZR2019QH006 and JQ201721);the Project of Shandong Province Higher Educational Science and Technology Program(No.J17KA228);the Innovation Team Project of Jinan Science&Technology Bureau(No.2018GXRC003);the Young Taishan Scholars Program(No.tsqn20161037);Shandong Talents Team Cultivation Plan of University Preponderant Discipline(No.10027).

摘　　要：Chemical investigation of the culture extract of an endophytic Penicillium citrinum from Dendrobium officinale,afforded nine citrinin derivatives(1–9)and one peptide-polyketide hybrid GKK1032B(10).The structures of these compounds were determined by spectroscopic methods.The absolute configurations of 1 and 2 were determined for the first time by calculation of electronic circular dichroism(ECD)data.Among them,GKK1032B(10)showed significant cytotoxicity against human osteosarcoma cell line MG63 with an IC50 value of 3.49μmol·L–1,and a primary mechanistic study revealed that it induced the apoptosis of MG63 cells via caspase pathway activation.

关 键 词：Penicillium citrinum ENDOPHYTIC GKK1032B ANTI-PROLIFERATION APOPTOSIS 

分 类 号：R284.1[医药卫生—中药学] R965[医药卫生—中医学]