干旱胁迫咖啡嫁接苗内源激素及生物量的变化  

Effect of Drought Stress on Endogenous Hormone Content and Biomass of Coffee Grafted Seedlings

作　　者：董云萍[1] 林兴军[1] 黄丽芳[1] 谭军 李金芹 赵青云[1] 黎秀元[1] 龙宇宙[1] DONG Yunping;LIN Xingjun;HUANG Lifang;TAN Jun;LI Jinqin;ZHAO Qingyun;LI Xiuyuan;LONG Yuzhou(Spice and Beverage Research Institute,Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Genetic Re-sources Utilization of Spice and Beverage Crops,Ministry of Agriculture and Rural Affairs/Key Laboratory of Genetic Improve-ment and Quality Regulation for Tropical Spice and Beverage Crops of Hainan Provincial,Wanning,Hainan 571533,China;School of Tropical Crops,Yunnan Agricultural University,Pu’er,Yunnan 665099,China)

机构地区：[1]中国热带农业科学院香料饮料研究所/农业农村部香辛饮料作物遗传资源利用重点实验室/海南省热带香辛饮料作物遗传改良与品质调控重点实验室,海南万宁571533 [2]云南农业大学热带作物学院,云南普洱665099

出　　处：《热带作物学报》2022年第2期311-320,共10页Chinese Journal of Tropical Crops

基　　金：云南省董云萍专家工作站(No.202105AF150081);国家自然科学基金项目(No.31901469);中国热带农业科学院基本科研业务费(No.1630142021013)。

摘　　要：为探明咖啡嫁接苗应对干旱胁迫其内源激素变化对植株生长的影响,布置了盆栽试验。接穗为中粒种咖啡‘大丰1号’,砧木分别为大粒种咖啡‘查理种16号’(种间嫁接)和中粒种咖啡‘热研2号’(种内嫁接),3种水分处理:(1)正常淋水土壤含水量(soil moisture content,SMC),18%≤SMC≤25%,(2)轻度胁迫15%≤SMC≤17%,(3)重度胁迫10%≤SMC≤12%。6个试验处理分别为种间嫁接L_(1)、L_(2)、L_(3),种内嫁接C_(1)、C_(2)、C_(3)。结果表明:种内嫁接苗根系IAA、ABA、TZR、IP含量在正常和轻度水分处理下均显著高于种间嫁接苗;干旱处理下种间嫁接苗叶和根IAA、ABA、Zeatin、TZR,种内嫁接苗叶IAA、ABA、Zeatin升高正响应干旱胁迫,但种内嫁接苗根IAA、Zeatin、TZR为负干旱胁迫响应;2种嫁接苗根IBA含量对干旱胁迫响应不同,种间嫁接苗重度干旱显著低于正常和轻度干旱,种内嫁接苗干旱显著高于正常水分;2种嫁接苗叶片、根ABA含量,干旱显著高于正常水分,叶片ABA含量L_(2)、L_(3)比L_(1)高65.13%、218.52%,C_(2)、C_(3)比C_(1)高31.10%、89.15%。根ABA含量L_(2)、L_(3)是L_(1)的7.71倍、9.05倍,C_(2)、C_(3)是C_(1)的1.79倍、1.48倍;重度干旱种间和种内嫁接苗叶面积损失率显著增加,单株生物量分别比正常水分减少23.45%、20.14%;轻度水分胁迫种间嫁接苗根干重、比根长、比根表面积分别比正常水分高39.16%、33.81%、28.07%,根系变细,根冠比0.66显著高于正常水分0.48;相关分析表明干旱胁迫下咖啡嫁接苗内源激素变化与生物量间存在相关性,地上部生物量与叶片IAA、Zeatin、ABA和根ABA呈显著、正负相关,根直径与根IAA、TZR、IP、ABA呈显著负相关,侧根数与根IAA、Zeatin、TZR、IP、ABA呈显著正相关。综上,在正常和轻度干旱下种内嫁接苗根系生长强于种间嫁接苗,IBA对调控种内嫁接苗根系应对干旱有积极作用,ABA对2种咖啡嫁接苗耐受干旱起关键作用,轻A pot experiment was conducted to study the response mechanism of endogenous hormones and plant growth of the grafted coffee seedlings under drought stress.Coffea canephora var.Dafeng No.1 was used as the scion,C.liberica var.Dewevrei No.16 and C.canephora var.Reyan No.2 as the rootstocks.The treatment included well-watered,soil moisture content (SMC),18%≤SMC≤25%,light water stress,17%≤SMC≤15%,severe water stress,10%≤SMC≤12%.There were three interspecific grafting,L_(1),L_(2),L_(3) and three intraspecific grafting,C_(1),C_(2),C_(3).IAA,ABA,TZR,IP in the root for the intraspecific grafted seedlings showed higher than those for the interspecific grafted seedlings under well-watered and light water stress.IAA,ABA,Zeatin and TZR in the leaves and roots of the interspecific grafted seedlings and IAA,ABA and Zeatin in the leaves of the intraspecific grafted seedlings responded positively to drought stress,but IAA,Zeatin and TZR in the roots of the intraspecific grafted seedlings responded negatively to drought stress.The IBA content in the roots of two grafted seedlings had different responses to drought stress.The IBA content in the roots of the interspecific grafted seedlings under severe drought was significantly lower than that under well-watered and light drought,while the IBA content in the roots of the intraspecific grafted seedlings under drought treatment was significantly higher than that under well-watered.ABA in the leaves and roots in both grafted seedlings increased significantly under drought stress.ABA in the leaves of L_(2) and L_(3) was 65.13%,218.52%higher than that of L_(1),ABA in the leaves of C_(2) and C_(3) was 31.10%,89.15%higher than that of C_(1).ABA in the roots L_(2) and L_(3) was7.71,9.05 times higher than that of L_(1),ABA in the roots of C_(2) and C_(3) was 1.79,1.48 times higher than that of C_(1).Under severe water stress,the leaf area loss rate significantly increased,the biomass in treatment L and C decreased 23.45%,20.14%compared with the well-watered.Under light water st

关 键 词：咖啡 嫁接苗 干旱胁迫 内源激素 

分 类 号：S571.2[农业科学—作物学]