胃癌患者血清IL-1的升高和癌组织JAK2/STAT3信号通路的激活  

Increased serum IL-1 level and activated JAK2/STAT3 signaling pathway of cancer tissue in patients with gastric cancer

作　　者：牛世伟 苏韫 龚红霞 李婷婷 曾元丁 张晗 聂蓬 NIU Shi-wei;SU Yun;GONG Hong-xia;LI Ting-ting;ZENG Yuan-ding;ZHANG Han;NIE Peng(School of Basic Medicine,Gansu University of Traditional Chinese Medicine,Lanzhou 730000;Provincial-Level Key Laboratory for Molecular Medicine of Major Diseases and Study on Prevention, Gansu University of Traditional Chinese Medicine, Lanzhou 730000;Department of Gastric Surgery,Wuwei Academy of Medical Sciences, Gansu Province Wuwei Cancer Hospital, Wuwei 733000, China)

机构地区：[1]甘肃中医药大学基础医学院,甘肃兰州730000 [2]甘肃中医药大学甘肃省高校重大疾病分子医学与中医药防治研究省级重点实验室,甘肃兰州730000 [3]甘肃省武威肿瘤医院武威医学科学院胃外科,甘肃武威733000

出　　处：《基础医学与临床》2022年第3期401-405,共5页Basic and Clinical Medicine

基　　金：国家自然科学基金(81660744);甘肃省中医方药挖掘与创新转化重点实验室基金(ZYFY-2020-004);甘肃中医药大学中西医结合基础学科科研培育项目[(5)2020—2021年度];敦煌医学与转化教育部重点实验室基金(DHYX19-08)。

摘　　要：目的探讨胃癌患者血清中白细胞介素1(IL-1)含量升高及癌组织中JAK2/STAT3信号通路激活在胃癌生长过程中的作用。方法选取甘肃省武威肿瘤医院胃癌手术患者及同期健康体检者各30例;酶联免疫吸附法(ELISA)检测样本血清中IL-1和IL-10的含量;免疫组化和Western blot分别检测各组织中Bcl-2、Bax及JAK2、STAT3、pJAK2、pSTAT3、Bcl-2和Bax的含量。实时荧光定量PCR检测各组织中JAK2、STAT3、Bcl-2和Bax基因的含量。结果与对照组相比,胃癌患者血清中IL-1含量上升,而IL-10含量下降(P<0.05);Bcl-2在癌组织中的含量明显高于对照组和癌旁组,而Bax含量相反(P<0.05)。与对照组相比,癌组织和癌旁组织中JAK2、STAT3、pJAK2、pSTAT3和Bcl-2蛋白含量增加,而Bax含量减少(P<0.05)。与癌旁组织相比,癌组织中JAK2、STAT3、pJAK2、pSTAT3和Bcl-2蛋白含量增加,而Bax含量减少(P<0.05)。与对照组相比,癌组织和癌旁组织中JAK2、STAT3和Bcl-2 mRNA含量增加,而Bax mRNA含量减少(P<0.05)。与癌旁组织相比,癌组织中JAK2、STAT3和Bcl-2 mRNA含量增加,而Bax mRNA含量减少(P<0.05)。结论促炎因子IL-1的异常升高及JAK2/STAT3信号通路的异常激活与胃癌的发生发展关系密切。Objective To explore the role of elevated serum interleukin 1(IL-1)levels in patients with gastric cancer and activation of JAK2/STAT3 signaling pathway in tissues of gastric cancer.MethodsA total of 30 gastric cancer surgery patients and 30 healthy subjects were selected from Wuwei Cancer Hospital of Gansu Province;The levels of IL-1 and IL-10 in serum was determined by enzyme-linked immunosorbent assay(ELISA);Immunohistochemistry and Western blot were used to detect the contents of Bcl-2,Bax and JAK2,STAT3,pJAK2,pSTAT3,Bcl-2,Bax in tissues.The contents of JAK2,STAT3,Bcl-2 and Bax genes were measured by RT-qPCR.ResultsCompared with the control group,the level of IL-1 in the serum of patients with gastric cancer was up-regulated,and the level of IL-10 was down-regulated(P<0.05);The content of Bcl-2 in cancer tissue was significantly higher than that in the normal group and the adjacent group,but the Bax content showed an opposite profile(P<0.05).Compared with the control group,JAK2,STAT3,pJAK2,pSTAT3 and Bcl-2 protein content in cancer tissue and adjacent tissues were increased,while the content of Bax was decreased(P<0.05).Compared with the adjacent tissues,the JAK2,STAT3,pJAK2,pSTAT3 and Bcl-2 protein contents in cancer tissues increased,while the Bax content was decreased(P<0.05).Compared with the control group,the contents of JAK2,STAT3 and Bcl-2 mRNA in cancer tissues and adjacent tissues were increased,while Bax mRNA was decreased(P<0.05).Compared with adjacent tissues,the contents of JAK2,STAT3 and Bcl-2 mRNA in cancer tissues increased,while the content of Bax mRNA was decreased(P<0.05).Conclusions Abnormal elevation of proinflammatory factors and abnormal activation of JAK2/STAT3 signaling pathway are closely related to the occurrence and development of gastric cancer.

关 键 词：胃癌 JAK2/STAT3信号通路 IL-1 IL-10 

分 类 号：R735.2[医药卫生—肿瘤]