罗哌卡因抑制人肝癌细胞系Hep3B增殖、迁移和侵袭  

Ropivacaine inhibits proliferation,migration and invasion of human liver cancer cell line Hep3B

作　　者：孙全鹏 樊超[2] 展德玺 范怡明 孙伟峰 SUN Quan-peng;FAN Chao;ZHAN De-xi;FAN Yi-ming;SUN Wei-feng(Depatrment of Anesthesiology, the First Affiliated Hospital of Zhengzhou University, Zhengzhou 450044;Depatrment of Anesthesiology, Zhengzhou Orthopedic Hospital, Zhengzhou 450044;Depatrment of Anesthesiology,Dancheng People's Hospital, Zhoukou 477150, China)

机构地区：[1]郑州大学第一附属医院麻醉科,河南郑州450044 [2]郑州市骨科医院麻醉科,河南郑州450044 [3]郸城县人民医院麻醉科,河南周口477150

出　　处：《基础医学与临床》2022年第3期448-453,共6页Basic and Clinical Medicine

基　　金：河南省医学科技攻关计划省部共建项目(201701035)。

摘　　要：目的研究罗哌卡因对人肝癌细胞系Hep3B生物学行为的影响,并探讨其分子机制。方法设置对照组、罗哌卡因组、si-NC组、si-LINC00853组、pcDNA-NC组、pcDNA-LINC00853组、(罗哌卡因+pcDNA-NC)组、(罗哌卡因+pcDNA-LINC00853)组。采用细胞计数试剂盒(CCK-8法)、Transwell小室法、RT-qPCR检测细胞增殖、迁移、侵袭以及LINC00853表达。Western blot分析细胞增殖核抗原(PCNA)、基质金属蛋白酶2(MMP2)和MMP9蛋白的表达水平。结果与对照组比较,罗哌卡因组Hep3B细胞增殖抑制率升高,迁移和侵袭细胞数减少,PCNA、MMP2和MMP9以及LINC00853表达显著降低(P<0.05)。与si-NC组比较,si-LINC00853组Hep3B细胞增殖抑制率升高,迁移和侵袭细胞数减少,PCNA、MMP2和MMP9蛋白表达降低(P<0.05)。与pcDNA-NC组比较,pcDNA-LINC00853组Hep3B细胞增殖抑制率降低,迁移和侵袭细胞数增多,PCNA、MMP2和MMP9蛋白表达升高(P<0.05)。与(罗哌卡因+pcDNA-NC)组比较,(罗哌卡因+pcDNA-LINC00853)组Hep3B细胞增殖抑制率降低,迁移和侵袭细胞数增多,PCNA、MMP2和MMP9蛋白表达升高(P<0.05)。结论罗哌卡因可抑制肝癌细胞系Hep3B的增殖、迁移和侵袭,其机制可能与下调LINC00853表达有关。Objective To study the effects of ropivacaine on the biological behaviour of liver cancer cell line Hep3B and to explore its molecular mechanism.Methods Hep3B cells were divided into the control group,ropivacaine group,si-NC group,si-LINC00853 group,pcDNA-NC group,pcDNA-LINC00853 group,(ropivacaine+pcDNA-NC)group and(ropivacaine+pcDNA-LINC00853)group.Cell Counting Kit(CCK-8),Transwell assays,and RT-qPCR were applied to detect cell proliferation,migration,invasion and LINC00853 expression.Western blot was used to analyze the expression of proliferating cell nuclear antigen(PCNA),matrix metalloproteinase 2(MMP2)and MMP9 proteins.Results Compared with the NC group,the proliferation inhibition rate of Hep3B cells in the ropivacaine group was elevated,cell migration and invasion were inhibited.The expression of PCNA,MMP2,MMP9 and LINC00853 was reduced(P<0.05).Compared with the si-NC group,the proliferation inhibition rate of Hep3B cells in the si-LINC00853 group was elevated,cell migration and invasion were reduced and the expression of PCNA,MMP2,MMP9 protein was decreased(P<0.05).Compared with the pcDNA-NC group,the proliferation inhibition of Hep3B cells in the pcDNA-LINC00853 group was reduced,cell migration and invasion were increased.The expression of PCNA,MMP2,and MMP9 protein was increased(P<0.05).Compared with the(ropivacaine+pcDNA-NC)group,the proliferation inhibitio of Hep3B cells in the(ropivacaine+pcDNA-LINC00853)group was reduced,cell migration and invasion were increased and the expression of PCNA,MMP2 and MMP9 protein increased(P<0.05).Conclusions Ropivacaine can inhibit liver cancer cell line Hep3B proliferation,migration and invasion.Its mechanism may be related to the down-regulation of LINC00853 expression.

关 键 词：罗哌卡因 LINC00853 肝癌 增殖 迁移 侵袭 

分 类 号：R735.7[医药卫生—肿瘤]