通络益气方对博来霉素致大鼠肺纤维化的治疗作用研究  

Studies on Therapeutic Effect of Tongluo Yiqi Recipe on Bleomycin-induced Pulmonary Fibrosis in Rats

作　　者：蒋鹏娜 张莹莹[2] 江柏华[1] 张佩青 张碧海 李星 JIANG Peng-na;ZHANG Ying-ying;JIANG Bai-hua;ZHANG Pei-qing;ZHANG Bi-hai;LI Xin(Heilongjiang Academy of Traditional Chinese Medicine,Harbin,Heilongjiang,150036,China;The First Affiliated Hospital of Heilongjiang University of Traditional Chinese Medicine,Harbin,Heilongjiang,150024,China)

机构地区：[1]黑龙江省中医药科学院,黑龙江哈尔滨150036 [2]黑龙江中医药大学附属第一医院,黑龙江哈尔滨150040

出　　处：《现代生物医学进展》2022年第1期52-56,共5页Progress in Modern Biomedicine

基　　金：黑龙江省自然科学基金重点项目(ZD2018019);黑龙江省博士后面上资助项目(LBH-Z19100)。

摘　　要：目的:研究通络益气方对博来霉素致大鼠模型肺组织病理及肺组织羟脯氨酸(HYP)的影响。方法:将36只SPF级健康6周龄Wistar雄性大鼠置于SPF级条件下,适应性试养3天后,分为3组:空白组、模型组、治疗组,每组12只。除空白组外,其余两组,在清醒状态下,放入与小动物雾化给药仪相连的30 cm×30 cm×20 cm自制玻璃箱中,雾化吸入浓度为5 g/L(50%)的博莱霉素,雾化20分钟,休息5分钟,一天连续激发2小时,连续刺激4周。空白组在同样条件下雾化吸入0.9%生理盐水,操作方法与其余两组相同。治疗组大鼠造模后第2天起给予通络益气方,通络益气方溶液按体质量给予大鼠药液1 m L/100 g灌胃,每次于雾化前1h灌胃;模型组、正常组均给予生理盐水(1 m L/100 g)灌胃。各组大鼠连续刺激4周后,用1%戊巴比妥钠(50 mg/kg)麻醉后,待其肌肉松驰、呼吸平稳后,仰面固定于专用板上,用动物专用CT进行肺扫描。各组大鼠在末次诱导后,24 h内腹腔注射1%戊巴比妥钠麻醉后,取肺组织进行HE和Masson染色,观察各组大鼠肺组织病理HE染色、Masson染色及肺组织中HYP的含量。结果:与空白组(0.76±0.06)相比,治疗组(1.11±0.13)、模型组(1.47±0.22)HYP均升高(P<0.05);与模型组(1.47±0.22)相比,治疗组(1.11±0.13)HYP明显降低(P<0.05)。结论:通络益气方能降低对肺纤维化大鼠肺组织中胶原的沉积,起到治疗肺纤维化的作用,与通络益气方抑制HYP胶原蛋白的表达有关。Objective: To study the effect of Tongluo Yiqi Recipe on lung tissue pathology and lung tissue hydroxyproline(HYP)in a rat model induced by bleomycin. Method(s): 36 SPF-grade healthy 6-week-old Wistar male rats were placed under SPF-grade conditions. After 3 days of adaptive trial rearing, they were divided into 3 groups: blank group, model group, and treatment group, with12 rats in each group. Except for the blank group, the other two groups were awake and placed in a 30 cm×30 cm×20 cm self-made glass box connected to the small animal nebulization dosing device. The inhalation concentration was 5 g/L(50%). Lymycin, sprayed for 20 minutes, rested for 5 minutes, stimulated continuously for 2 hours a day, stimulated continuously for 4 weeks. The blank group inhaled0.9% saline under the same conditions, and the operation method was the same as that of the other two groups. The rats in the treatment group were given Tongluo Yiqi Recipe from the 2 nd day after modeling, and the Tongluo Yiqi Recipe solution was given to the rats by gavage of 1 m L/100 g of liquid medicine according to their body weight, each time 1 hour before nebulization;Both the model group and the normal group were given saline(1 m L/100 g) by gavage. After 4 weeks of continuous stimulation, rats in each group were anesthetized with 1% sodium pentobarbital(50 mg/kg), and after their muscles were relaxed and breathing was stable, they were fixed on their backs on a special board, and the lungs were scanned by animal CT. After the last induction, rats in each group were anesthetized by intraperitoneal injection of 1% sodium pentobarbital within 24 hours, and then lung tissues were taken for HE and Masson staining to observe the pathological HE staining, Masson staining and the content of HYP in the lung tissues of rats in each group. Results:Compared with the blank group, the HYP of the treatment group and the model group were increased(P<0.05);compared with the model group, the HYP of the treatment group was significantly lower(P<0.05). Conclusion

关 键 词：通络益气方 中医药 动物模型 肺纤维化 羟脯氨酸 

分 类 号：R-33[医药卫生] R563