Zn和Cd胁迫作用对长梗白菜形态和生理性能的生态毒理影响  

Ecotoxicological effects of Zn and Cd stress on the morphology and physiological properties of long-stalk cabbage

作　　者：张小茜 张富贵 董祥林[4] 魏祥平[4] 郑刘根[1,2] ZHANG Xiaoxi;ZHANG Fugui;DONG Xianglin;WEI Xiangping;ZHENG Liugen(School of Resources and Environmental Engineering,Anhui University,Hefei,230601,China;Anhui Province Engineering Laboratory for Mine Ecological Remediation,Hefei,230601,China;The Scientific Research and Design Institute of Environmental Protection ofZibo,Zibo,255040,China;Huaibei Mining(Group)Co.,Ltd.Anti-piping and Measuring Department,Huaibei,235000,China)

机构地区：[1]安徽大学资源与环境工程学院,合肥230601 [2]安徽省矿山生态修复工程实验室,合肥230601 [3]淄博市环境保护科学研究设计院,淄博255040 [4]淮北矿业(集团)有限责任公司通防地测部,淮北235000

出　　处：《环境化学》2022年第1期22-30,共9页Environmental Chemistry

基　　金：安徽高校协同创新项目(GXXT-2019-029);平安煤炭开采工程技术研究院有限责任公司科技项目(W2018JSFW0551);国家自然科学基金(41702176)资助.

摘　　要：本文以不同Cd、Zn浓度胁迫下的长梗白菜为研究对象,采用盆栽实验,分析了Cd和Zn胁迫下长梗白菜形态参数(重量、长度、表面积、叶的分形维数)、生理性能的影响以及从土壤中提取Cd和Zn的能力。结果表明,Cd、Zn胁迫对长梗白菜的生长具有“低促高抑”效应,且共同胁迫对生长的影响大于单一胁迫。在0.6 mg·kg^(-1) Cd和250 mg·kg^(-1) Zn同时胁迫下叶茎生物量分别增加93.27%和155.84%;当Cd、Zn同时胁迫下浓度分别升至0.9 mg·kg^(-1)和350 mg·kg^(-1),叶茎生物量分别降低63%和66.23%。随重金属浓度的升高,植物中过氧化物酶(POD)的活性先升高后降低。与对照组相比,在150 mg·kg^(-1) Zn胁迫下POD活性提高了64.17%,在350 mg·kg^(-1) Zn胁迫下降低了31.50%。重金属处理导致长梗白菜中过氧化氢酶(CAT)活性不同程度升高。在0.9 mg·kg^(-1) Cd和350 mg·kg^(-1) Zn同时胁迫下达到最大活性99.74 U·g^(-1)。Cd和Zn的联合处理显著降低了丙二醛(MDA)含量。Zn的添加促进Cd的吸收,根中Cd含量可达0.21 mg·kg^(-1)。研究结果可为土壤重金属复合污染防治,提高蔬菜品质提供科学支撑。In this paper,the long-stalk cabbage under different Cd and Zn concentration stresses was used as the research object.Potted experiments were used to analyze the morphological parameters(length,weight,surface area,leaf fractal dimension)and physiological properties of long-stemmed cabbage under Cd and Zn stress.Influence and the ability to extract Cd and Zn from the soil.The results showed that:Cd and Zn stress have a“low-promoting high-inhibition”effect on the growth of long-stemmed cabbage,and the common stress has a greater impact on the growth than a single stress.Under the simultaneous stress of 0.6 mg·kg^(-1) Cd and 250 mg·kg^(-1) Zn,the leaf and stem biomass increased by 93.27%and 155.84%,respectively;when Cd and Zn were simultaneously stressed,the concentration increased to 0.9 mg·kg^(-1) and 350,respectively mg·kg^(-1),the leaf and stem biomass decreased by 63%and 66.23%,respectively.As the concentration of heavy metals increases,the activity of peroxidase(POD)in plants first increases and then decreases.Compared with the control group,POD activity increased by 64.17%under 150 mg·kg^(-1) Zn stress,and decreased by 31.50%under 350 mg·kg^(-1) Zn stress.The treatment of heavy metals resulted in the increase of CAT activity in long-stemmed cabbage.The maximum activity of 99.74 U·g^(-1) was reached under the simultaneous stress of 0.9 mg·kg^(-1) Cd and 350 mg·kg^(-1) Zn.The combined treatment of Cd and Zn significantly reduced the content of malondialdehyde(MDA).The addition of Zn promotes the absorption of Cd,and the Cd content in the root can reach 0.21 mg·kg^(-1).The research results can provide scientific support for the prevention and control of soil heavy metal compound pollution and the improvement of vegetable quality.

关 键 词：CD ZN 氧化应激 重金属复合作用 长梗白菜 

分 类 号：X173[环境科学与工程—环境科学]