响应面法优化三叉苦黄酮提取工艺及抗氧化活性分析  

Optimization of Extraction Process of Total Flavonoids from Melicope Pteleifolia and Evaluation of Its Antioxidant Activity

作　　者：罗桂花 林翠清 肖鸬华 方媛 莫穗芬 杨惠琳 严萍 詹若挺[1,2] LUO Gui-hua;LIN Cui-qing;XIAO Lu-hua;FANG Yuan;MO Sui-fen;YANG Hui-lin;YAN Ping;ZHAN Ruo-ting(School of Chinese Medicine,Guangzhou University of Chinese Medicine,Guangzhou 510006,China;Chinese Medicine Resources Science and Engineering Research Center,Key Laboratory of Lingnan Traditional Chinese Medicine Resources Ministry of Education(Guangzhou University of Chinese Medicine),Guangzhou 510006,China)

机构地区：[1]广州中医药大学中药学院,广东广州510006 [2]广州中医药大学中药资源科学与工程研究中心,岭南中药资源教育部重点实验室(广州中医药大学)国家中成药工程技术研究中心南药研发实验室,广东广州510006

出　　处：《食品与药品》2022年第1期1-6,共6页Food and Drug

基　　金：广东省普通高校“服务乡村振兴计划”重点领域专项—基于广东乡村振兴的南药产业科技服务体系构建(编号:2019KZDZX2017);广东省省级乡村振兴战略专项—广东省现代南药产业技术体系创新团队(编号:2020KJ148)。

摘　　要：目的采用星点设计-响应面法优化三叉苦总黄酮提取工艺,并考察抗氧化活性。方法在单因素考察结果的基础上,对三叉苦总黄酮提取工艺中乙醇体积分数(%)、液料比(ml/g)、加热回流提取时间(min)3个因素进行优化,得到较优的总黄酮提取工艺。通过测定三叉苦总黄酮DPPH和ABTS自由基清除能力评价其抗氧化活性。结果三叉苦总黄酮最佳提取工艺:乙醇体积分数90%,液料比10 ml/g,提取时间30 min,该条件下实际的提取率2.99%,与预测值3.25%偏差较小,清除DPPH和ABTS自由基能力较强。结论运用响应面设计得到的方法稳定可靠,可用于三叉苦总黄酮的提取。三叉苦有显著的抗氧化能力,为其进一步开发和应用提供一定依据,在功能食品和药品开发方面具有较好前景。Objective To optimize the extraction process of total flavonoids from Melicope pteleifolia by central composite design(CCD)and response surface methodology,and evaluate the antioxidant activity.Methods Based on single factor tests,the ethanol volume fraction(%),the liquid-solid ratio(ml/g)and extraction time(min)were optimized by CCD method.The antioxidant activity of extraction from the Melicope pteleifolia was evaluated by DPPH and ABTS assays.Results The optimal extraction parameters of total flavonoids from Melicope pteleifolia were as follows:the ethanol volume fraction was 90%,the ratio of liquid to material was 10 ml/g and the extraction time was 30 min.Under this optimal condition,the yield of total flavonoids was 2.99%,which had a small deviation from the predicted value of 3.25%.The total flavonoids had strong scavenging ability to DPPH free radical and ABTS.Conclusions The extraction method of the total flavonoids from Melicope pteleifolia is stable and efficient.The extract has significant antioxidant capacity that provides a basis for the further development and application in the field of functional foods and drugs.

关 键 词：三叉苦 总黄酮 星点设计-响应面法 加热回流法 抗氧化活性 

分 类 号：R283.6[医药卫生—中药学]