膜联蛋白A7低表达对人胃癌MGC-803细胞增殖和凋亡的影响  

Effects of Low Expression of ANXA7 on Proliferation and Apoptosis of Human Gastric Cancer MGC-803 Cells

作　　者：刘明伟 柏友兰 王小杰 李欣 LIU Ming-wei;BAI You-lan;WANG Xiao-jie;LI Xin(School of Basic Medicine,Chengde Medical University,Hebei,067000,China)

机构地区：[1]承德医学院基础医学院,河北承德067000

出　　处：《承德医学院学报》2022年第1期5-9,共5页Journal of Chengde Medical University

基　　金：河北省教育厅项目(Z2014026,ZD2018058);河北省科技厅项目(10276142);承德医学院校级课题(201712)。

摘　　要：目的探讨膜联蛋白A7(ANXA7)低表达对人胃癌MGC-803细胞增殖和凋亡的影响。方法将人胃癌MGC-803细胞分为ANXA7低表达组(si-ANXA7)和阴性对照组(si-con),分别将靶向ANXA7的si-RNA和阴性对照RNA转染为ANXA7低表达组和阴性对照组。Western blot法检测ANXA7的表达;MTT法检测各组细胞的增殖能力;PI检测各组细胞的凋亡率;Western blot检测各组细胞增殖和凋亡相关蛋白PCNA和Cleaved casepase-3的表达。结果与阴性对照组相比,ANXA7低表达组MGC-803细胞增殖能力显著降低,增殖相关蛋白PCNA表达显著下降(P<0.05);细胞凋亡增强,相关蛋白Cleaved casepase-3的表达显著升高(P<0.05)。结论ANXA7低表达可抑制人胃癌MGC-803细胞的增殖并促进其凋亡。Objective To investigate the effects of low-expression of annexin A7(ANXA7)on the proliferation and apoptosis of human gastric cancer MGC-803 cells.Methods Human gastric cancer MGC-803 cells were divided into ANXA7 low-expression group(si-ANXA7),negative control group(si-con).Si-RNA targeting ANXA7 and negative control RNA were transfected into ANXA7 low-expression group and negative control group respectively.The expression of ANXA7 was detected by Western blot;MTT assay was used to detect the proliferation of cells in each group;The apoptosis rate in each group was detected by PI;The expressions of PCNA and Cleaved casepase-3 were detected in each group by Western blot.Results Compared with the negative control group,the proliferation ability of MGC-803 cells in the low-expression group of ANXA7 decreased significantly,and the expression of proliferation related proteins PCNA decreased significantly(P<0.05);The expression of cleaved casepase-3 increased significantly(P<0.05).Conclusion The low-expression of ANXA7 may inhibitthe proliferation and promote the apoptosis of human gastric cancer MGC-803 cells.

关 键 词：ANXA7 MGC-803细胞 增殖 凋亡 

分 类 号：R735.2[医药卫生—肿瘤]