GP30 of the mycobacteriophage CASbig impairs mycobacterial adaptation during acidic stress and in macrophages  

作　　者：WU LI JIANGZHE SI SHUAI QIU JING LUO HUIQIONG SUN XIAOQING LI ZHIBIN WAN WEI GAO HANLU ZOU LEI ZHANG XIAOHONG XIANG YANZHANG LI TIESHAN TENG 

机构地区：[1]Key Laboratory of Regional Characteristic Agricultural Resources,College of Life Sciences,Neijiang Normal University,Neijiang,641100,China [2]School of Medical Sciences,College of Medicine,Henan University,Kaifeng,475004,China [3]School of Pharmacy,Chongqing Medical and Pharmaceutical College,Chongqing,401331,China

出　　处：《BIOCELL》2020年第4期695-701,共7页生物细胞（英文）

基　　金：Key R&D and Promotion Projects of Henan Province,grant number 192102310156,the National Natural Science Foundation of China,Grant No.81601740;the Applied Basic Research Program of Science&Technology Department of Sichuan Province,Grant No.2018JY0108;the National Undergraduate Training Program for Innovation and Entrepreneurship(X2017013,X2019070,202010475006).

摘　　要：The rapid emergence of multidrug-resistant and extensively drug-resistant Tuberculosis retrieved intense interest in phage-based therapy.This old approach,which was abandoned in the west in the 1940s but is generating renewed interest,has stimulated fresh research on mycobacteriophages and their lytic efficiency against their hosts.GP30 is a novel protein of the mycobacteriophage CASbig with undiscovered function.In this study,we analyzed the role of CASbig gp30 in the host Mycobacterium smegmatis.Overexpression of gp30 in the host led to reduced growth in acidic medium and attenuated the intracellular survival rate of M.smegmatis inside the THP-1 macrophages,which may be linked to the altered lipid profile of the recombinant bacterial cell wall.In a word,this study suggested that gp30,a novel gene from a mycobacteriophage,modulated lipid composition and content to hamper the survivability of bacteria under stress conditions.

关 键 词：MYCOBACTERIUM SMEGMATIS gp30 CASbig ACIDIC STRESS Macrophage 

分 类 号：TP3[自动化与计算机技术—计算机科学与技术]