Phytohormonal and metabolism analysis of Brassica rapa L.ssp.pekinensis with different resistance during Plasmodiophorabrassicae infection  

作　　者：MEI LAN JINGFENG HU HONGLI YANG LIQIN ZHANG XUEZHONG XU JIANGMING HE 

机构地区：[1]Institute of Horticultural Crops,Yunnan Academy of Agricultural Sciences,Yunnan Branch of the National Vegetable Improvement Center,Kunming,650205,China

出　　处：《BIOCELL》2020年第4期751-767,共17页生物细胞（英文）

基　　金：the National Bulk Vegetable Industry Technology System of China(CARS-23-G37);Research and integrated demonstration of green key technologies of main export vegetables in Yunnan Province(2019ZG001);Yunnan Province“ten thousand talents plan”Yunling industrial technology leading talents,Special support of Modern Agricultural Technology Department of vegetables in Yunnan Province.The funder has no role in study design,data collection and analysis,decision to publish,or preparation of the manuscript.

摘　　要：Clubroot of Chinese cabbage(Brassica rapa L.ssp.pekinensis),caused by the obligate parasite Plasmodiophora brassicae,accounts for serious yield losses.The aim of our study was to explore the phytohormone levels and metabolome changes in the roots of resistant and susceptible B.rapa genotypes at a late stage of infection,i.e.,28 days post-infection.Both genotypes showed decreased auxin levels after P.brassicae infection except for indole-3-acetic acid.Overall,the susceptible genotype had higher auxin and cytokinin levels after infection,with the exception of trans-zeatin and 3-indolebutyric acid as compared to the resistant genotype.Jasmonic acid levels declined after infection regardless of the genotype.Resistance against clubroot was evident with the increased levels of salicylic acid in the resistant genotype.The susceptible genotype had a higher number of differentially accumulated metabolites(DAMs)(262)than the resistant genotype(238)after infection.Interestingly,132 DAMs were commonly detected in both genotypes when infected with the pathogen,belonging to metabolite classes such as phenolic acids,amino acids,and derivatives,glucosinolates,organic acids,flavonoids,nucleotides and derivatives,and fatty acids.The differential metabolite analysis revealed that metabolites related to amino acid biosynthesis,fatty acid biosynthesis and elongation,glutathione metabolism,and glucosinolate metabolism were highly accumulated in the resistant genotype,suggesting their essential roles in resistance against P.brassicae infection.

关 键 词：AUXINS Brassicaceae CLUBROOT CYTOKININS Fatty acids GLUCOSINOLATES Glutathione Salicylic ACID Jasmonic ACID 

分 类 号：O62[理学—有机化学]