An in vitro study to explore the role of prolylcarboxypeptidase in non-small cell lung cancer  

作　　者：Binbin QIAN Xiaoduo LIU Xiaolin GU Lu YANG Dake CHEN 

机构地区：[1]Department of Oncology,Nantong Tongzhou People’s Hospital,Nantong,226300,China [2]Department of Laboratory Medicine,Nantong Tongzhou People’s Hospital,Nantong,226300,China [3]Department of Obstetrics and Gynaecology,Nantong Tongzhou People’s Hospital,Nantong,226300,China

出　　处：《BIOCELL》2020年第1期19-26,共8页生物细胞（英文）

基　　金：Scientific research projects of Nantong Health and Family Planning Commission(No.WKZD2018032).

摘　　要：Prolylcarboxypeptidase(PRCP)belongs to the S28 family of proteases,which is also a dipeptidyl peptidase.In this study,we demonstrate the expression pattern of PRCP in Non-small cell lung cancer(NSCLC).We found that the repression of PRCP expression by small interfering RNA successfully inhibited cell proliferation,migration,and invasion.Further,we explored the involvement of PRCP in the regulation of epithelial-mesenchymal transition(EMT).The epithelial marker E-cadherin was significantly increased,meanwhile mesenchymal markers MUC1,vimentin,and SNAIL were markedly decreased in PRCP knockdown cells.Moreover,the downregulation of PRCP in the NSCLC cells induced the expression of apoptosis-related proteins in vitro.We performed RT-PCR in 30 pairs of clinical NSCLC tissues and adjacent non-cancerous tissues,which revealed significantly higher PRCP expression levels in cancer tissues than in adjacent non-cancerous tissues.Collectively the results from our study suggest a possible cancer promotion role of PRCP in NSCLC.

关 键 词：APOPTOSIS Cell lines EMT NSCLC PRCP 

分 类 号：R73[医药卫生—肿瘤]