Effect of peroxiredoxin 6 on total and progressive motility of human spermatozoa after cryopreservation  

作　　者：TIECHENG SUN LUYANG CHENG JING MA SHANJIE ZHOU YANDONG ZHANG WENDI ZHOU SHOULONG DENG SHUSONG WANG TIAN LI 

机构地区：[1]Reproductive Medical Center,Department of Obstetrics and Gynecology,Peking University International Hospital,Beijing,102206,China [2]Department of Immunology,Basic Medical College,Chengde Medical University,Hebei,067000,China [3]NHC Key Laboratory of Family Planning and Healthy/Key Laboratory of Reproductive Medicine of Hebei Province,Shijiazhuang,050051,China [4]Beijing Key Laboratory for Animal Genetic Improvement,National Engineering Laboratory for Animal Breeding,Key Laboratory of Animal Genetics and Breeding of the Ministry of Agriculture,College of Animal Science and Technology,China Agricultural University,Beijing,100193,China [5]CAS Key Laboratory of Genome Sciences and Information,Beijing Institute of Genomics,Chinese Academy of Sciences,Beijing,100101,China [6]Reproductive Medicine Centre,Peking University Second Affiliated Hospital,Beijing,100044,China

出　　处：《BIOCELL》2020年第3期323-327,共5页生物细胞（英文）

基　　金：Peking Post-doctoral Research Fund(EE 2019-50);Peking University International Hospital Research Funds(No.YN2019QN13);NHC Key Laboratory of Family Planning and Healthy/key laboratory of reproductive medicine of Hebei provincial(SZ-202006).

摘　　要：Sperm cryopreservation is useful in assisted reproductive technology and male fertility preservation.However,freezing and thawing significantly reduces the total and progressive motility of human spermatozoa.In the present study,we explored the effects of peroxiredoxin 6(PRDX6)on total and progressive motility of human spermatozoa after cryopreservation.Semen samples of 20 males with normal parameters were collected and frozen in media supplemented with different concentrations of PRDX6(0 mM,10−5 mM,10−7 mM,and 10−9 mM,respectively).Postthaw total and progressive motility of sperms were measured.The results showed that in comparison with 0 mM,the concentrations of 10−5 mM,10−7 mM,and 10−9 mM of PRDX6 all significantly improved total motility and progressive motility of sperms(p<0.05).The 10−7 mM of PRDX6 showed the best performance.In conclusion,the supplementation of PRDX6 helps to maintain the total and progressive motility of human spermatozoa.

关 键 词：PEROXIREDOXIN 6 CRYOPRESERVATION SPERM MOTILITY PROGRESSIVE MOTILITY 

分 类 号：R33[医药卫生—人体生理学]