除硫菌剂的工业化生产条件研究与应用  

Study and application of industrialized production conditions of sulfur removal bacteria

作　　者：杨帆[1,2] 孟章进 姜桂英[1] 钱志鸿 王志明 YANG Fan;MENG ZhangJin;JIANG Guiying;QIAN Zhihong;WANG Zhiming(Petroleum Engineering Technology Research Institute of Jiangsu Oilfield Company,Yangzhou 225009,China;Jiangsu Oil and Gas Microbial Engineering Research Center,Yangzhou 225009,China)

机构地区：[1]中国石化江苏油田分公司工程技术研究院,江苏扬州225009 [2]江苏省油气微生物工程研究中心,江苏扬州225009

出　　处：《复杂油气藏》2021年第4期116-120,共5页Complex Hydrocarbon Reservoirs

摘　　要：除硫菌N411是一种可以高效除硫的高温反硝化细菌,为提高该菌的培养效能,提高发酵水平,开展了工业发酵培养基优化研究和工业生产条件研究。通过调整培养基中碳和氮的比例,对基础培养基进行优化,从4组配方中优化出最优培养基,考察不同温度、pH值和装液量对除硫菌生长状况的影响,从而确定工业发酵条件。根据确定的发酵条件生产的除硫菌剂,经平板活菌计数法测定的菌数可达2.2×10^(8) CFU/mL。Desulfurization bacteria N411 is a kind of high-temperature denitrifying bacteria that can remove sulfur efficiently.In order to improve the culture efficiency and fermentation level of N411,it was carried out studies on the optimization of industrial fermentation medium and production conditions.The basic medium was optimized by adjusting the proportion of carbon and nitrogen in the medium,and the optimal medium was obtained from the four sets of formulas.The effects of temperature,pH value,and liquid volume on the growth of sulfur removal bacteria were investigated to determine industrial fermentation conditions.According to the sulfur removal bacteria produced under the determined fermentation conditions,the number of bacteria can reach 2.2×10^(8) CFU/mL by the plate viable bacteria counting method.

关 键 词：除硫菌 培养基 工业发酵 

分 类 号：Q939.97[生物学—微生物学]