外周血G6PD活性检测对于感染高危型人乳头瘤病毒宫颈癌患者的诊断预后价值  

Potential Value of G6PD Activity Detection in the Peripheral Blood for the Diagnosis and Prognosis of Cervical Cancer Patients with High Risk Human Papillomavirus Infection

作　　者：陈红兰 吴怡[3] 胡滔 王玉明[1] CHEN Honglan;WU Yi;HU Tao;WANG Yuming(Dept.of Laboratory Medicine,Second Affiliated Hospital of Kunming Medical University,Kunming Yunnan 650101;Dept.of Laboratory Medicine,The Third People’s Hospital of Yunnan Province,Kunming Yunnan 650200;Science and Technology Achievement Incubation Center,Kunming Medical University,Kunming Yunnan 650500,China)

机构地区：[1]昆明医科大学第二附属医院医学检验科,云南昆明650101 [2]云南省第三人民医院医学检验科,云南昆明650200 [3]昆明医科大学科技成果孵化中心,云南昆明650500

出　　处：《昆明医科大学学报》2022年第2期89-95,共7页Journal of Kunming Medical University

基　　金：云南省卫生和计划生育委员会医学后备人才培养计划资助项目(H-2017026)。

摘　　要：目的探讨外周血葡萄糖-6-磷酸脱氢酶(Glucose-6-phosphate dehydrogenase,G6PD)活性检测对于感染高危型人乳头瘤病毒(high-risk Human papilloma virus,HR-HPV)宫颈癌患者的诊断预后价值。方法选取2014年6月至2015年12月间确诊的宫颈癌患者53例、宫颈上皮内瘤病变(cervicalintraepithelial neoplasia,CIN)患者58例、健康体检者40例为研究对象,采用HPV检测试剂盒对各组人群HPV感染进行检测与基因分型;宫颈癌患者手术切除标本进行病理学评估及分型;同时取各组人群血清标本,运用免疫荧光法检测G6PD的活性;结合临床资料及G6PD活性检测结果进行相关分析;并对检测结果进行循证分析。结果宫颈癌组中外周血G6PD的活性最高,与CIN组、健康对照组相比有统计学差异(P<0.01),CIN组与健康对照组相比,差异具有统计学意义(P<0.05);CIN患者外周血中G6PD的活性改变与患者的疾病病程恶化密切相关;宫颈癌组与CIN组外周血中G6PD的活性都与患者HPV16/18型感染的状态契合;HPV 16/18感染宫颈癌患者OS曲线与外周血G6PD活性的关联性分析结果显示,外周血G6PD活性高的患者5 a生存率显著下降(P<0.05)。HPV 16/18感染检测与外周血G6PD活性检测在CIN及宫颈癌的循证实验诊断中价值较高,其中G6PD活性检测对于CIN的诊断价值优于HPV16/18检测。结论G6PD活性与宫颈癌患者病程进展相关,外周血G6PD活性检测可作为高危型HPV尤其是HPV 16/18型感染宫颈癌的早期诊断及预后指标之一。Objective To explore the value of detecting G6PD activity in the peripheral blood for the diagnosis and prognosis of cervical cancer patients with high-risk human papillomavirus(HR-HPV)infection Methods A total of 53 cervical cancer(CC)patients,58 cervical intraepithelial neoplasia(CIN)patients and 40 health cohorts(HC),visiting and attending the clinic of Yunnan Third People’s Hospital during June 2014 to December 2015,were employed in this study.HPV Gene Chip Test Kit was used to detect and screen the genotype HPV infection in each group of population.Pathological evaluation and genotyping were performed on surgical specimens of cervical cancer patients.Serum samples were taken from each group of population,the activity of G6PD was detected by immunofluorescence assay,and the correlation analysis was carried out according to the clinical data and the results of G6PD activity.The results were then analyzed based on evidence.Results The activity of G6PD in peripheral blood was the highest in CC group,and there was significant difference to CIN Group and HC Group(P<0.01),and CIN Group to HC Group(P<0.05).The change of G6PD activity in peripheral blood of CC patients is closely related to the progression of cervical cancer,and the change of G6PD activity in peripheral blood of CIN patients is closely related to the progression of cervical cancer.In CC group and CIN Group,the activity of G6PD in peripheral blood was consistent with HPV16/18 infection,and the relationship between the OS curve and the activity of G6PD in peripheral blood of CC patients infected with HPV 16/18 was analyzed,the 5-year survival rate decreased significantly in patients with high G6PD activity in peripheral blood(P<0.05).The validity and reliability of G6PD activity detection and/or HPV16/18 infection test are valuable in evidence-based diagnosis for CIN or cervical cancer.Conclusions The activity of G6PD is related to the progression of cervical cancer.The detection of G6PD activity in peripheral blood can be used as one of the diagnost

关 键 词：G6PD 高危型人乳头瘤病毒 宫颈癌 预后 循证诊断 

分 类 号：R446.11[医药卫生—诊断学]