盐芥叶片应答盐胁迫的蛋白质组学分析  

Proteomics analysis of Eutrema salsugineum leaves in response to salt stress

作　　者：常丽丽[1] 彭存智[1] 王丹[1] 仝征[1] 黄超[1] 徐兵强[1,2] CHANG Li-li;PENG Cun-zhi;WANG Dan;TONG Zheng;HUANG Chao;XU Bing-qiang(Institute of Tropical Bioscience and Biotechnology,Chinese Academy of Tropical Agricultural Sciences,Haikou 571101,China;Haikou Experimental Station,Chinese Academy of Tropical Agricultural Sciences,Haikou 571101,China)

机构地区：[1]中国热带农业科学院热带生物技术研究所,海南海口571101 [2]中国热带农业科学院海口实验站,海南海口571101

出　　处：《江苏农业学报》2022年第1期49-64,共16页Jiangsu Journal of Agricultural Sciences

基　　金：中央级公益性科研院所基本科研业务费项目(1630052017008);海南省重点研发计划项目(ZDYF2018180)。

摘　　要：盐芥是研究耐盐机理的模式植物。为从蛋白质水平揭示盐芥响应盐胁迫的分子机制,本研究采用同位素标记相对和绝对定量(iTRAQ)技术对不同NaCl浓度处理7 d的盐芥叶片进行差异蛋白质组学分析。结果表明,盐芥叶片中共鉴定到4607个蛋白质,其中281个蛋白质的表达丰度显著增加,95个蛋白质的表达丰度显著降低。盐胁迫差异表达蛋白质的KEGG代谢通路和蛋白质互作网络分析结果表明,促进植株光合作用可帮助盐芥适应低盐环境;抑制叶绿素和支链氨基酸合成、调控应激反应基因的表达是盐芥应对中盐环境的重要因素;而有效清除活性氧、提高渗透物质积累量和增加能量供应可能是盐芥耐受高盐环境的关键。本研究结果为揭示盐芥应答盐胁迫的分子响应机制提供了理论基础。Eutrema salsugineum is a model plant for salt tolerance study.To reveal the molecular mechanism of E.salsugineum in response to salt stress at protein level,isobaric tags for relative and absolute quantification(iTRAQ)technology was used to do differential proteomics analysis on E.salsugineum leaves treated under different NaCl concentrations for seven days.The results showed that,a total of 4607 proteins were identified from leaves of E.salsugineum,among which the expressional abundance of 281 proteins were significantly up-regulated,while the expressional abundance of 95 proteins were significantly down-regulated.Results of KEGG metabolic pathway and protein-protein interaction network analysis for differentially expressed proteins under salt stress showed that,promotion of photosynthesis was helpful for E.salsugineum to adapt to environment with low salinity.Inhibition of chlorophyll and branched chain amino acids synthesis,as well as expression regulation of stress-responsive genes were important factors for E.salsugineum to survive in an environment with medium salinity.Moreover,the key points for E.salsugineum to tolerate high salinity condition might be effective elimination of reactive oxygen species(ROS),elevating accumulation of osmolytes and increasing energy supply.The results can provide theoretical basis for revealing molecular mechanism of E.salsugineum in response to salt stress.

关 键 词：盐芥 盐胁迫 差异蛋白质 ITRAQ 

分 类 号：R73[医药卫生—肿瘤]