经大鼠尾动脉置鞘法建立介入通路的研究  

Establishment of interventional access via the sheathing of the caudal artery in rats

作　　者：高群 胡深[1] 樊静雯 王大明[1] GAO Qun;HU Shen;FAN Jingwen(Beijing Hospital,Beijing 100730,China)

机构地区：[1]北京医院,北京100730

出　　处：《中风与神经疾病杂志》2022年第2期135-138,共4页Journal of Apoplexy and Nervous Diseases

基　　金：国家自然科学基金面上项目(82071329)。

摘　　要：目的由于缺乏连续动脉内操作的方法,数字剪影血管造影术在啮齿类动物模型中的应用受到了限制。我们旨在探究通过单一鞘管通路对大鼠尾动脉进行重复介入操作的方法。方法雄性Sprague Dawley大鼠(650~700 g)6只,全身麻醉后显露尾动脉,以显微剪将血管做“鱼嘴形”切口后,通过导丝指引18G鞘管建立尾动脉介入通道;通道建立完成后,经尾动脉鞘管通路分别行大鼠全脑血管造影以及球囊、支架导管主动脉扩张术,检测尾动脉置鞘法作为介入通路的可行性及有效性。结果大鼠尾动脉直径约(1.19±0.14)mm,导丝指引下顺利植入18G鞘管;与颈动脉(24.50±4.51)min及股动脉(18.17±3.43)min置鞘时间相比,尾动脉置鞘时间明显减少(11.00±1.90 min,P<0.01)。经尾动脉鞘管通道成功完成大动脉及全脑血管造影;球囊导管以及支架导管可顺利通过鞘管通道并在主动脉释放,释放完成后可顺利退出鞘管;每只大鼠尾动脉可供完成多次置鞘操作,且单一鞘管通道建立后可重复进行血管造影及介入操作。结论经尾动脉介入通道建立法适用于大鼠血管内造影、治疗以及构建动物模型。这种方法进一步提高了大鼠作为人类疾病模型的应用范围。Objective Digital silhouette angiography in animal models is limited by the lack of methods that can perform continuous intra-arterial manipulations.We aimed to investigate the way that allowed to repeat interventional maneuvers through a single sheath access in the rat caudal artery.Methods Six adult male Sprague Dawley rats(650~700 g)were anesthetized,the caudal arteries were exposed and a fish-mouth-shaped incision was made with a microscissor.After the access was established,whole brain angiography and aortic dilatation with balloon and stent catheters were performed via the tail artery sheath access to test the feasibility and effectiveness of the tail artery sheathing method as an interventional access.Results The diameter of caudal artery approximately(1.19±0.14)mm,and the 18G sheath was successfully implanted with guidewire.The sheathing time of the caudal artery(11.00±1.90 min,P<0.01)was significantly reduced compared with the carotid artery(24.50±4.50)min and the femoral artery(18.17±3.43)min.A balloon catheter and a stent catheter could be passed through the caudal artery sheath and released in the aorta,and the catheter could be withdrawn smoothly after the release.Each rat can be used for multiple tail artery sheathing operations,and repeatable angiographic and interventional procedures can be performed through a single sheath channel.Conclusions The method of establishing interventional access using a sheath introduced through the rat tail artery is suitable for intravascular imaging,treatment,and the construction of rat animal models.This method further enhances the scope of application of rats as human disease models.

关 键 词：介入通道 尾动脉 数字剪影血管造影术 动物模型 

分 类 号：R741[医药卫生—神经病学与精神病学]