小檗科科尔切斯淫羊藿叶绿体全基因组研究  

Complete chloroplast genome of Epimedium pinnatum Fisch.ex DC.(Berberidaceae)

作　　者：于淑霞 梁琼[2,3] 罗惠[2] 黄若琪 李永 张燕君[2,3,4] Yu Shu-Xia;Liang Qiong;Luo Hui;Huang Ruo-Qi;Li Yong;Zhang Yan-Jun(Innovation Platform of Molecular Biology,College of Forestry,Henan Agricultural University, Zhengzhou 450002, China;Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture,Wuhan Botanical Garden,Chinese Academy of Sciences, Wuhan 430074, China;Center of Conservation Economic Botany,Core Botanical Gardens,Chinese Academy of Sciences, Wuhan 430074, China;University of Chinese Academy of Sciences, Beijing 100049, China)

机构地区：[1]河南农业大学林学院分子生物学创新平台,郑州450002 [2]中国科学院武汉植物园,中国科学院植物种质创新与特色农业重点实验室,武汉430074 [3]中国科学院核心植物园资源植物学协同中心,武汉430074 [4]中国科学院大学,北京100049

出　　处：《植物科学学报》2022年第1期39-46,共8页Plant Science Journal

基　　金：国家自然科学基金(31670346,32071675);中国科学院武汉植物园重点科研人才项目(Y855281A05);武汉市科学技术局应用基础前沿项目(2019020701011435)。

摘　　要：小檗科科尔切斯淫羊藿(Epimedium pinnatum Fisch.ex DC.)是分布于高加索地区的一种具有观赏和药用价值的多年生草本植物。本研究首次报道了科尔切斯淫羊藿的叶绿体全基因组序列。结果显示:科尔切斯淫羊藿叶绿体基因组全长为156155 bp,GC含量为38.82%;由一个大的单拷贝区(LSC,89425 bp)、一个小的单拷贝区(SSC,16284 bp)和一对反向重复区(IRa和IRb,25223 bp)组成。叶绿体基因组包含112个基因,其中蛋白质编码基因78个,tRNA基因30个,rRNA基因4个。系统发育分析结果表明,Rhizophyllum亚属的科尔切斯淫羊藿与Epimedium亚属的Macroceras组最先聚为一支,并与Epimedium亚属的Diphyllon组形成的一支分开。Epimedium pinnatum Fisch.ex DC.(Berberidaceae)is a perennial herb with ornamental and medicinal value distributed in the Caucasus region.In this study,the Illumina NovaSeq 6000 sequencing system was used to assemble the complete chloroplast genome sequence of E.pinnatum for the first time.Results showed that the total length of the chloroplast genome was 156155 bp with 38.82%GC content,comprising a large single-copy region(LSC,89425 bp),small single-copy region(SSC,16284 bp),and pair of inverted repeat regions(IRa and IRb,25223 bp).The chloroplast genome of E.pinnatum contained 112 genes,including 78 protein-coding genes,30 transfer RNA(tRNA)genes,and four ribosomal RNA(rRNA)genes.Phylogenetic analysis showed that E.pinnatum subgen.Epimedium and sect.Macroceras was clustered on a branch,which was separated from the branch composed of species from sect.Diphyllon,subgen.Epimedium.These results suggest that further study is needed to clarify the natural system of Epimedium based on more data.

关 键 词：科尔切斯淫羊藿 叶绿体基因组 小檗科 

分 类 号：Q943.2[生物学—植物学]