STIL在神经胶质瘤中表达及对增殖能力的影响  

Expression of STIL in glioma and its effect on proliferation

作　　者：钟锰[1] 王晓娇[1] 王君 刘畅[1] 朱彬[1] 王鹤[3] 李英夫[3] 何伟明[1] ZHONG Meng;WANG Xiao-Jiao;WANG Jun(Department of Neurosurgery,Affiliated Hospital of Inner Mongolia University for Nationalities,Tongliao 028007,Inner mongolia,China)

机构地区：[1]内蒙古民族大学附属医院神经外科,内蒙古通辽028007 [2]内蒙古医科大学基础医学院 [3]佳木斯大学第一附属医院神经外科

出　　处：《中国老年学杂志》2022年第5期1158-1162,共5页Chinese Journal of Gerontology

基　　金：内蒙古自然科学基金(2020MS08029)。

摘　　要：目的探讨STIL在神经胶质瘤中表达及生物学意义。方法公用数据库TCGA在线分析STIL在神经胶质瘤中表达水平,并检测了20例神经胶质瘤临床标本中STIL表达量,利用siRNA干扰技术瞬时敲低胶质瘤细胞系中STIL的表达水平,并利用定量聚合酶链反应(qPCR)及Western印迹检测敲低效率,免疫组织化学染色(SP)法和Western印迹检测STIL的敲低效率和对神经胶质瘤细胞增殖的影响。结果与正常组织相比,SITL在神经胶质瘤组织中显著高表达,与TCGA数据库分析结果一致。TCGA数据库分析显示STIL高表达与神经胶质瘤患者低生存期相关。与人正常星形胶质细胞NHA中mRNA相比,STIL在细胞系U251、SHG-44、SWO-38细胞中均有表达,在细胞系U87细胞中表达最高,sh-STIL-1组SHG-44细胞和SWO-38细胞STIL蛋白水平均显著低于sh-NC组(P<0.05)。第2天后,sh-STIL-1组SHG-44细胞和SWO-38细胞增殖能力均显著低于Sh-NC组(P<0.05)。通过免疫荧光实验分析发现敲低STIL的表达水平后,紫外诱导胶质瘤细胞SWO-38内产生基因组DNA双链断裂数目明显增多,导致基因组不稳定的发生,敲低STIL的表达导致p27蛋白水平上调。结论敲低STIL表达能有效抑制神经胶质瘤细胞的增殖能力。神经胶质瘤中STIL高表达与临床分期呈正相关,并参与细胞周期及DNA修复等过程,有望成为神经胶质瘤诊疗的新分子靶标。Objective To investigate the expression and biological significance of STIL in glioma.Methods The public database TCGA was used to analyze the expression level of STIL in glioma online,and the expression level of STIL in 20 glioma clinical specimens was detected,and the expression level of STIL in glioma cell line was transiently knocked down by siRNA interference technology,real-time polymerase chain reaction(q-PCR)and Western blotting were used to detect the knockdown efficiency,and immunohistochemical staining(S-P method)and Western blotting were used to detect the knockdown efficiency of STIL and its effect on the proliferation of glioma cells.Results Compared with normal tissues,SITL was significantly higher expressed in glioma tissues,consistent with the TCGA database analysis results.TCGA database analysis showed that high STIL expression was associated with poor survival in glioma patients.Compared with mRNA in human normal astrocytes NHA,STIL was expressed in cell lines U251,SHG-44,U87,SWO-38 cells,and the highest expression was in cell line U87.The STIL protein levels of SHG-44 cells and SWO-38 cells in sh-STIL-1 group were significantly lower than those in sh-NC group(P<0.05).After the second day,the proliferation ability of SHG-44 cells and SWO-38 cells in STIL-1 group were significantly lower than those in sh-NC group(P<0.05).Immunofluorescence analysis showed that after knocking down the expression level of STIL,the number of genomic DNA double-strand breaks in the UV-induced glioma cells SWO-38 were significantly increased,resulting in the occurrence of genomic instability,and knocking down the expression of STIL resulted in the level of p27 protein raised.Conclusions Knockdown of STIL expression could effectively inhibit the proliferation ability of glioma cells.The high expression of STIL in gliomas is positively correlated with clinical stage,and participates in cell cycle and DNA repair processes.It is expected to be a new molecular target for the diagnosis and treatment of glioma.

关 键 词：STIL 胶质瘤 基因组稳定性 

分 类 号：R739.41[医药卫生—肿瘤]