斑菲素蛋白3在胰腺癌中的表达及其与肿瘤增殖、迁移的关系  

Expression of plakophilin 3 in pancreatic cancer and its relationship with tumor proliferation and migration

作　　者：赵栓柱 刘攀 禹鹏飞 李文磊 付强[2] 张旭 罗乾坤 秦涛[2] Zhao Shuanzhu;Liu Pan;Yu Pengfei;Li Wenlei;Fu Qiang;Zhang Xu;Luo Qiankun;Qin Tao(Department of Hepatobiliary and Pancreatic Surgery,People’s Hospital of Zhengzhou University,Zhengzhou 450003,China;Department of Hepatobiliary and Pancreatic Surgery,Henan Provincial People’s Hospital,Zhengzhou 450003,China)

机构地区：[1]郑州大学人民医院肝胆胰腺外科,450003 [2]河南省人民医院肝胆胰腺外科,郑州450003

出　　处：《中华实验外科杂志》2022年第1期14-17,共4页Chinese Journal of Experimental Surgery

基　　金：河南省省部共建项目(SB201901083);河南省科技攻关项目(212102310151、192102310391)。

摘　　要：目的观察斑菲素蛋白3(PKP3)在胰腺癌中的表达,探讨PKP3对胰腺癌细胞增殖、迁移能力的影响。方法利用在线生信分析网路数据库基因表达谱交互分析(GEPIA)观察PKP3在胰腺癌中的表达水平;收集2019年4月至2021年4月就诊于郑州大学人民医院行手术治疗的25例胰腺癌组织及其相应的正常组织,实时荧光定量聚合酶链式反应(qRT-PCR)检测组织中PKP3的表达水平;蛋白质印迹法(Western blot)检测人胰腺腺癌细胞(SW1990,BXPC3)、人正常胰腺导管上皮细胞(HPDE)中PKP3的表达水平。小干扰RNA构建PKP3低表达细胞株,分为siPKP3-1、siPKP3-2组和空白对照组(NC-PKP3)。细胞计数试剂盒(CCK-8)实验检测胰腺癌细胞的增殖能力,划痕愈合实验、Transwell实验检测其迁移能力,两组间比较采用t检验。结果PKP3在胰腺癌组织中表达量为(2.32±0.93),明显高于胰腺正常组织(1.57±0.42,t=9.246,P<0.01),差异有统计学意义,Western blot结果表明PKP3在胰腺癌细胞BXPC3中的表达量高于正常胰腺细胞HPDE[(1.74±0.15)倍],在胰腺癌细胞SW1990中的表达量高于正常胰腺细胞HPDE[(1.34±0.08)倍]。CCK-8实验提示转染72 h后,BXPC3细胞中siPKP3-1、siPKP3-2组吸光度值分别为(2.09±0.37、1.67±0.42),明显低于NC-PKP3组(3.66±0.67,t=10.683、11.361,P<0.01),差异有统计学意义;SW1990细胞中siPKP3-1、siPKP3-2组吸光度值分别为(2.36±0.45、1.68±0.39),明显低于NC-PKP3组(3.54±0.98,t=7.664、8.727,P<0.01),差异有统计学意义;转染后24 h划痕愈合实验结果表明BXPC3细胞中siPKP3-1、siPKP3-2组划痕愈合百分比分别为(36.14±4.77)%、(22.56±5.15)%,明显低于NC-PKP3组[(78.61±5.03)%,t=9.375、9.716,P<0.01];SW1990细胞中siPKP3-1、siPKP3-2组划痕愈合百分比分别为(18.03±3.85)%、(23.12±5.02)%,明显低于NC-PKP3组[(68.33±6.98)%,t=9.772、9.913,P<0.01];Transwell实验结果表明,SW1990细胞中siPKP3-1、siPKP3-2组的细胞穿膜数量为(33.23±4.26)、(35.93±5.76)个/视野Objective To observe the expression of plakophilin 3(PKP3)in pancreatic cancer,and to discuss the effect of PKP3 on the proliferation and migration of pancreatic cancer cells.Methods The online biometric analysis network database gene expression profiling interactive analysis(GEPIA)was used to observe the expression level of PKP3 in pancreatic cancer.A total of 25 cases of pancreatic cancer tissues and their corresponding normal tissues that were treated in our hospital from April 2019 to April 2021 were collected.Real-time fluorescence quantitative polymerase chain reaction(qRT-PCR)was used to detect the expression level of PKP3 in tissues.Western blotting was used to detect the expression of PKP3 in SW1990,BXPC3 human pancreatic cancer cell lines,and human normal pancreatic ductal epithelial cells(HPDE).The small interfering RNA was used to construct PKP3 low-expressing cell lines,which were divided into siPKP3-1,siPKP3-2 and negative control(NC-PKP3)groups.The cell counting kit-8(CCK-8)assay was used to detect the proliferation ability of pancreatic cancer cells,and the scratch healing experiment and the Transwell experiment was used to detect the migration ability.The t test was used for comparison between groups.Results The expression level of PKP3 in pancreatic cancer tissues was(2.32±0.93),which was significantly higher than that in normal pancreatic tissues(1.57±0.42,t=9.246,P<0.01).Western blotting results showed that the expression of PKP3 in pancreatic cancer cells BXPC3 was higher than in HPDE[(1.74±0.15)times],and the expression in SW1990 cells was higher than in pancreatic cell HPDE[(1.34±0.08)times].The CCK-8 assay indicated that at 72 h after transfection,the absorbance values of siPKP3-1 and siPKP3-2 groups in BXPC3 cells were 2.09±0.37 and 1.67±0.42,respectively,which were significantly lower than in the NC-PKP3 group(3.66±0.67,t=10.683,11.361,P<0.01).The absorbance values of the siPKP3-1 and siPKP3-2 groups in SW1990 cells were 2.36±0.45 and 1.68±0.39,respectively,which were significan

关 键 词：斑菲素蛋白3 胰腺癌 增殖 迁移 

分 类 号：R735.9[医药卫生—肿瘤]