月季自交不亲和性S-RNase的鉴定与分析  

Identification and Analysis of Self Incompatibility S-RNase in Rose

作　　者：李茂福[1,2,3] 杨媛 王华[1,2,3] 范又维 孙佩[1,2,3] 金万梅 LI Maofu;YANG Yuan;WANG Hua;FAN Youwei;SUN Pei;JIN Wanmei(Institute of Forestry and Pomology,Beijing Academy of Agriculture and Forestry Sciences,Beijing 100093,China;Key Laboratory of Biology and Genetic Improvement of Horticultural Crops(North China),Ministry of Agriculture,Beijing 100093,China;Beijing Engineering Research Center of Functional Floriculture,Beijing 100093,China;Beijing Engineering Research Center for Deciduous Fruit Trees,Beijing 100093,China)

机构地区：[1]北京市农林科学院林业果树研究所,北京100093 [2]农业部华北地区园艺作物生物学与种质创制重点实验室,北京100093 [3]北京市功能花卉工程技术研究中心,北京100093 [4]北京市落叶果树工程技术研究中心,北京100093

出　　处：《园艺学报》2022年第1期157-165,共9页Acta Horticulturae Sinica

基　　金：北京市农林科学院科技创新能力专项(KJCX20200109,KJCX20200204,KJCX20200207);北京市林业果树科学研究院青年科研基金项目(LGYJJ202003)。

摘　　要：以月季‘月月红’(Rosa chinensis‘Slater’s Crimson China’)和‘2015-58-20’(Rosa sp.)为材料进行自交和异交,授粉花柱中花粉管荧光显微镜观察结果显示月季为典型的配子体自交不亲和植物。通过与已知蔷薇科自交不亲和S-RNase基因的序列进行比对,在‘月月红’中克隆到RcS_(1)-RNase和RcS_(2)-RNase基因的cDNA和DNA全长序列,序列分析表明,RcS_(1)-RNase和RcS_(2)-RNase具有5个保守结构域(C1、C2、C3、RC4和C5)和1个高变区。系统进化分析发现,RcS-RNase与其他蔷薇科植物的S-RNase蛋白具有较高的同源性。组织特异性表达分析表明,RcS^(1)-RNase和RcS_(2)-RNase基因只在花柱中特异表达。In Rosa chinensis‘Slater’s Crimson China’and Rosa sp.‘2015-58-20’,the fluorescence microscope observation of pollen tubes in both self-pollinated and cross-pollinated styles indicated that rose is a typical gametophyte self-incompatible plant.The full-length sequence of RcS_(1)-RNase and RcS_(2)-RNase cDNA and DNA have been identified in the‘Slater’s Crimson China’.Sequence analysis showed that RcS_(1)-RNase and RcS 2-RNase have five conserved domains(C1,C2,C3,RC4 and C5)and a hypervariable region.Phylogenetic analysis found that RcS-RNase has high homology with S-RNase proteins in other Rosaceae plants.Tissue specific expression analysis indicated that RcS_(1)-RNase and RcS_(2)-RNase genes are solely expressed in styles.

关 键 词：月季 自交不亲和 S-RNASE 基因克隆 基因表达 

分 类 号：S685.12[农业科学—观赏园艺]