‘德钦’紫花苜蓿叶绿体基因组序列及特征分析  

Complete Chloroplast Genome Sequence and Characteristics Analysis of Medicago sativa‘Deqin’

作　　者：孙志轩 敖平星 毕玉芬[2] 赵雁[1] SUN Zhi-xuan;AO Ping-xing;BI Yu-fen;ZHAO Yan(College of Horticulture and Landscape, Yunnan Agricultural University, Kunming, Yunnan Province 650201, China;College of Animal Science and Technology, Yunnan Agricultural University, Kunming, Yunnan Province 650201, China)

机构地区：[1]云南农业大学园林园艺学院,云南昆明650201 [2]云南农业大学动物科学技术学院,云南昆明650201

出　　处：《草地学报》2022年第2期320-328,共9页Acta Agrestia Sinica

基　　金：国家自然科学基金项目(31660683);云南农业大学第十四届学生科技创新创业行动基金项目(2021ZKX086)资助。

摘　　要：‘德钦’紫花苜蓿(Medicago sativa L.‘Deqin’,以下简称‘德钦’苜蓿)是一种优良的地方品种,具有特异耐旱性、耐热性和耐酸铝性。本研究利用Illumina第二代高通量测序技术对其叶绿体全基因组进行测序(NCBI登录号:MN218692),并对其结构及特征进行分析。结果显示:‘德钦’苜蓿叶绿体基因组含有一个反向重复区,属于IR缺失型;总长为125470 bp,共有110个基因,其中蛋白质编码基因76个、tRNA 30个和rRNA 4个,缺失3个基因(rps16,rpl22和infA),总GC含量为33.9%;相对同义密码子使用度显示,‘德钦’苜蓿70.74%的密码子使用度>1,偏好以A和T结尾;共鉴定出115个SSRs位点,其中clpP基因鉴定出与紫花苜蓿不同SSRs位点;最大似然树结果显示‘德钦’苜蓿与紫花苜蓿聚在一起,支持率为75%。研究结果将对苜蓿属叶绿体基因组工程研究及‘德钦’苜蓿特异基因的进一步鉴定利用提供一定的参考价值。Medicago sativa‘Deqin’(hereinafter referred to as‘Deqin’)is an excellent alfalfa local variety with strong drought,heat,and aluminum-acid toxicity resistance.In this study,the whole chloroplast genome of‘Deqin’was sequenced by Illumina second-generation high-throughput sequencing technology(NCBI accession number:MN218692),and its structure and characteristics were analyzed.The results showed that the chloroplast genome of‘Deqin’,with a total length of 125470 bp,has one inverted repeat region and belongs to IR lacking clad.A total of 110 coding genes,including 76 protein-coding genes,30 tRNAs and 4 rRNAs,and 3 genes(rps16,rpl22 and infA)are absent,with an overall GC content of 33.9%.Relative synonymous codon usage showed that 70.74%of codons RSCU>1 in‘Deqin’,with preference ending with A and T.SSRs analysis identified 115 SSRs loci.Different SSRs loci from‘Deqin’were identified in the gene of clpP.The phylogenetic tree shows that‘Deqin’and M.sativa L.were clustered into one group with 75%support.The results have reference value for Medicago chloroplast genome engineering and further identification and utilization of specific genes of‘Deqin’.

关 键 词：‘德钦’紫花苜蓿 叶绿体基因组 高通量测序 特征分析 系统发育分析 

分 类 号：S541[农业科学—作物学]