长链非编码RNA TTY15通过海绵miR337-3p上调JAK2促进舌鳞状细胞癌的生长和转移  

Effects of long non-coding RNA TTTY15 on the growth and metastasis of squamous cell carcinoma of tongue by upregulate the expressionof JAK2

作　　者：张婷[1] 郑见宝[2] ZHANG Ting;ZHENG Jianbao(Outpatient Department,The First Affiliated Hospital of Xi’an Jiaotong University,Shaanxi,Xi’an 710061,China;不详)

机构地区：[1]西安交通大学第一附属医院门诊部,西安市710061 [2]西安交通大学第一附属医院普通外科,西安市710061

出　　处：《河北医药》2022年第2期165-169,175,共6页Hebei Medical Journal

基　　金：陕西省自然科学基础研究项目(编号:2019SF-065)。

摘　　要：目的探讨长链非编码RNA(LncRNA)TTTY 15在舌鳞状细胞癌生长和转移中的作用。方法采用定量实时聚合酶链反应(qRT-PCR)检测TTTY15、miR-337-3p、JAK2在舌鳞状细胞癌组织和细胞系中的表达,采用CCK-8法检测舌鳞状细胞癌细胞的增殖,采用Transwel法检测舌鳞状细胞癌细胞的迁移和侵袭,采用荧光素酶报告基因法进行验证TTTY15与miR-337-3p的相互作用。Western blot分析TTTY15和miR-337-3p对JAK2表达的影响。结果TTTY15在舌鳞状细胞癌样本中表达明显上调,而miR-337-3p则下调。TTTY15敲低可显著降低舌鳞状细胞癌细胞的增殖、迁移和侵袭,而miR-337-3p转染则有相反的效应。此外,TTTY15可通过海绵miR-337-3p并下调其表达,而且在舌鳞状细胞癌样本中TTTY15和miR-337-3p有负向调节的关系,而miR-337-3p可直接靶向调节JAK2从而调节舌鳞状细胞癌细胞生长。结论TTTY15通过靶向调节miR-337-3p/JAK2的表达在舌鳞状细胞癌中发挥促癌效应。Objective To investigate the effects of long-chain non-coding RNA(LncRNA)TTTY 15 on the growth and metastasis of tongue squamous cell carcinoma.Methods Quantitative real-time polymerase chain reaction(qRT-PCR)was used to detect the expression levels of TTTY15 and miR-337-3p in tongue squamous cell carcinoma(TSCC)tissues and cell lines.CCK-8 method was used to detect the proliferation of TSCC cells.Transwell method was used to detect the migration and invasion of TSCC cells.Luciferase reporter gene method was used to verify the interaction between TTTY15 and microRNA-337-3p.Moreover Western Blot was used to analyze the effects of TTTY15 and microRNA-337-3p on JAK2 expression.Results The expression levels of TTTY15 were significantly up-regulated in TSCC,however,the expression levels of microRNA-337-3p were down-regulated.TTTY15 knockdown could significantly reduce the proliferation,migration and invasion of TSCC cells,while microRNA-337-3p had opposite effects.In addition,TTTY15 and microRNA-337-3p had negative relationship in TSCC.MiR-337-3p could directly target JAK2 to regulate the proliferation,migration and invasion of TSCC cells.Conclusion TTTY15 plays a carcinogenic role in TSCC by targetedly regulating the expression of microRNA-337-3p/JAK2.

关 键 词：TTTY15 miR-337-3p JAK2 舌鳞状细胞癌 增殖 迁移 侵袭 

分 类 号：R739.86[医药卫生—肿瘤]