mNAP在细菌感染鉴别诊断中应用价值  

Application of m NAP in Differential Diagnosis of Bacterial Infection

作　　者：杨海燕[1] 陈新宽[1] 王加平[2] 胡明 张欢欢[1] 刘波[1] YANG Hai-yan;CHEN Xin-kuan;WANG Jia-ping;HU Ming;ZHANG Huan-huan;LIU Bo(Department of Laboratory,the First People's Hospital of Lianyungang,Lianyungang 222002,Jiangsu,China;Department of Laboratory,Donghai County People's Hospital,Donghai 222300,Jiangsu,China)

机构地区：[1]连云港市第一人民医院检验科,江苏连云港222002 [2]东海县人民医院检验科,江苏东海222300

出　　处：《医学信息》2022年第4期118-120,共3页Journal of Medical Information

摘　　要：目的探讨中性粒细胞膜碱性磷酸酶(mNAP)在细菌、病毒感染鉴别诊断中价值。方法选择2019年6月-2020年6月连云港市第一医院住院经明确诊断的细菌感染患者124例,病毒感染患者48例,非感染患者40例(对照组)为研究对象。采用荧光标记流式细胞术检测mNAP,免疫散射比浊法检测CRP,通过ROC曲线评价mNAP、CRP诊断效能。结果细菌感染患者mNAP、CRP水平高于病毒感染患者和对照组(P<0.01);病毒感染患者m NAP水平与对照组比较,差异无统计学意义(P>0.05),而CRP水平高于对照组(P<0.01);mNAP、CRP诊断细菌感染AUC分别为0.980、0.970,诊断的敏感性分别为95.98%、94.87%;特异性分别为96.51%、74.23%。结论 mNAP在对细菌感染诊断特异性明显高于CRP,对细菌、病毒感染鉴别诊断具有一定参考价值。Objective To investigate the value of membrane neutrophil alkaline phosphatase(mNAP) in the differential diagnosis of bacterial and viral infection.Methods A total of 124 patients with bacterial infection,48 patients with viral infection and 40 patients without infection(control group)were selected from June 2019 to June 2020 in the First People’s Hospital of Lianyungang.Fluorescence labeled flow cytometry was used to detect mNAP,and immunoturbidimetry was used to detect CRP.ROC curve was used to evaluate the diagnostic efficiency of mNAP and CRP.Results The levels of mNAP and CRP in patients with bacterial infection were higher than those in patients with viral infection and control group( P<0.05);there was no significant difference in mNAP level between viral infection patients and control group( P >0.05),while CRP level was higher than that of control group(P <0.01).The AUC of mNAP and CRP in the diagnosis of bacterial infection was 0.980 and 0.970 respectively,the sensitivity was95.98% and 94.87% respectively,and the specificity was 96.51% and 74.23%,respectively.Conclusion The specificity of m NAP in the diagnosis of bacterial infection is significantly higher than CRP,which has a certain reference value for the differential diagnosis of bacteria and virus infection.

关 键 词：中性粒细胞膜碱性磷酸酶 C-反应蛋白 细菌感染 病毒感染 流式细胞 

分 类 号：R363[医药卫生—病理学]