枸杞多糖对肝纤维化大鼠血液及肝组织中IGF-2和IGFBP-2表达的影响  

Effects of Lycium barbarum polysaccharide on the expression of IGF-2 and IGFBP-2 in blood and liver tissue of hepatic fibrosis rats

作　　者：高深[1] 霍丽民[1] 韩艳珍[2] 栗志英[2] 任海霞[2] 单铁英[3] GAO Shen;HUO Limin;HAN Yanzhen;LI Zhiying;REN Haixia;SHAN Tieying(Handan First Hospital,Handan 056002;Affiliated Hospital of Hebei Engineering University,Handan 056002,Hebei,China;Medical School of Hebei Engineering University,Handan 056002,Hebei,China)

机构地区：[1]邯郸市第一医院,河北邯郸056002 [2]河北工程大学附属医院,河北邯郸056002 [3]河北工程大学医学院,河北邯郸056002

出　　处：《现代中西医结合杂志》2022年第3期325-328,共4页Modern Journal of Integrated Traditional Chinese and Western Medicine

基　　金：河北省医学科学研究课题(20190964)。

摘　　要：目的观察枸杞多糖对肝纤维化大鼠血液及肝组织中胰岛素样生长因子-2(IGF-2)和胰岛素样生长因子结合蛋白-2(IGFBP-2)表达的影响,探讨枸杞多糖抑制肝纤维化的作用机制。方法取健康SD大鼠18只,采用胆总管结扎法制备肝脏纤维化大鼠模型,将造模成功大鼠随机分为模型组和枸杞多糖组各6只,另选取同品种的正常SD大鼠6只作为对照组。枸杞多糖组每日给予枸杞多糖30μg/g灌胃,模型组和对照组给予等量的生理盐水灌胃,均连续30 d。HE染色观察各组肝组织结构,酶联免疫吸附法检测血清中IGF-2和IGFBP-2水平,蛋白印迹法检测肝组织中IGF-2和IGFBP-2蛋白表达量。结果与对照组比较,模型组大鼠肝组织呈现出面积大小不一的坏死区域,汇管区结缔组织增生;血清IGF-2、IGFBP-2水平和肝组织中IGF-2、IGFBP-2蛋白表达量均明显升高(P均<0.05)。与模型组比较,枸杞多糖组大鼠肝组织中坏死区域减小,汇管区结缔组织成分减少;血清IGF-2、IGFBP-2水平和肝组织中IGF-2、IGFBP-2蛋白表达量均明显降低(P均<0.05)。结论枸杞多糖可以通过调节血液和肝组织中IGF-2和IGFBP-2的表达来减轻肝纤维化损伤。Objective It is to observe the effect of Lycium barbarum polysaccharide on the expression of insulin-like growth factor-2(IGF-2) and insulin-like growth factor-binding protein-2(IGFBP-2) in blood and liver tissue of hepatic fibrosis rats, and to explore the inhibition of hepatic fibrosis by Lycium barbarum polysaccharide. mechanism of action. Methods Eighteen healthy SD rats were selected, and the liver fibrosis rat model was prepared by common bile duct ligation. The successful model rats were randomly divided into the model group and the Lycium barbarum polysaccharide group, 6 rats in each group, and 6 normal SD rats of the same species were selected as a control group. The Lycium barbarum polysaccharide group was given intragastric administration of Lycium barbarum polysaccharide 30 μg/g every day, and the model group and the control group were given the same amount of normal saline by intragastric administration, all treated for 30 days. The structure of liver tissue in each group was observed by HE staining, the levels of IGF-2 and IGFBP-2 in serum were detected by enzyme-linked immunosorbent assay, and the protein expressions of IGF-2 and IGFBP-2 in liver tissue were detected by Western blotting. Results Compared with the control group, necrotic areas of different sizes, and connective tissue hyperplasia in the portal area were found in the liver tissue of the rats in the model group;the levels of serum IGF-2 and IGFBP-2 and the protein expressions of IGF-2 and IGFBP-2 in the liver tissue were significantly increased(P<0.05). Compared with the model group, the necrotic area in the liver tissue of the rats in the Lycium barbarum polysaccharide group and the components of the connective tissue in the portal area were decreased;the levels of serum IGF-2 and IGFBP-2 and the protein expressions of IGF-2 and IGFBP-2 in the liver tissue were significantly decreased(all P<0.05). Conclusion Lycium barbarum polysaccharide can alleviate liver fibrosis injury by regulating the expression of IGF-2 and IGFBP-2 in blood

关 键 词：肝纤维化 枸杞多糖 胰岛素样生长因子-2 胰岛素样生长因子结合蛋白-2 大鼠 

分 类 号：R-332[医药卫生]