低FODMAP饮食对溃疡性结肠炎模型大鼠TNF-α、IL-17表达的影响  

Effect of low FODMAP diet on the expression of TNF-αand IL-17 in rats with ulcerative colitis

作　　者：陈燕 黄益倩 童晓清[2] 章小艳 束龙[2] 郑培奋[1,2] CHEN Yan;HUANG Yiqian;TONG Xiaoqing;ZHANG Xiaoyan;SHU Long;ZHENG Peifen(Department of Gastroenterology,Zhejiang Hospital,Hangzhou 310013,China)

机构地区：[1]浙江医院消化内科,杭州310013 [2]浙江医院临床营养科,杭州310013

出　　处：《浙江医学》2022年第3期236-240,245,共6页Zhejiang Medical Journal

基　　金：浙江省自然科学基金项目(LY17H030008);浙江省中医药科研基金项目(2020ZB009);浙江省卫生厅一般项目(2017KY190)。

摘　　要：目的探讨低可发酵寡聚糖、双糖、单糖及多元醇(FODMAP)饮食对溃疡性结肠炎(UC)大鼠TNF-α、IL-17表达的影响。方法将40只SD大鼠按照随机数字表法分为高FODMAP组、低FODMAP组、模型组和空白对照组,每组10只。空白对照组不进行处理,其余3组均采用三硝基苯磺酸灌肠法制作UC大鼠模型。干预阶段,高FODMAP组及低FODMAP组分别给予定制鼠粮,且同时给予美沙拉嗪治疗;而空白对照组及模型组仅给予清洁级鼠粮。干预周期为14 d,分别比较干预期间不同时间段各组大鼠的一般情况、疾病活动指数(DAI)评分、结肠黏膜大体形态损伤评分、结肠黏膜组织病理学评分、结肠黏膜和血清TNF-α、IL-17水平。结果高FODMAP组、低FODMAP组和模型组大鼠DAI均明显高于空白对照组(均P<0.05);与模型组相比,低FODMAP组大鼠在干预的第7和14天时,DAI评分均明显下降(均P<0.05);而高FODMAP组大鼠则在干预的第14天时,DAI评分出现明显下降(P<0.05);与低FODMAP组相比,高FODMAP组大鼠在干预的第7、14天时DAI明显升高(均P<0.05)。高FODMAP组和模型组大体形态损伤评分均明显高于空白对照组(均P<0.05);与模型组比较,高FODMAP组和低FODMAP组大体形态损伤评分均明显降低(均P<0.05);与低FODMAP组相比,高FODMAP组大鼠大体形态损伤评分明显升高(P<0.05);而低FODMAP组与空白对照组大体形态损伤评分比较差异无统计学意义(P>0.05)。高FODMAP组、低FODMAP组和模型组组织病理学评分均明显高于空白对照组(均P<0.05);与模型组比较,高FODMAP组和低FODMAP组组织病理学评分均明显降低(均P<0.05)。与空白对照组相比,高FODMAP组、低FODMAP组和模型组结肠黏膜和血清TNF-α、IL-17水平均明显升高(均P<0.05);与模型组相比,高FODMAP组和低FODMAP组TNF-α、IL-17水平均明显降低(均P<0.05);与低FODMAP组相比,高FODMAP组结肠黏膜TNF-α、IL-17水平均明显升高(均P<0.05)。结论低FODMAP饮食�Objective To explore the effect of low FODMAP diet on the expression of TNF-αand IL-17 in ulcerative colitis(UC)rats.Methods Forty SD rats were divided into blank control group,model group,low FODMAP group and high FODMAP group according to randomnumber table method,with 10 rats in each group.The UC rat model was prepared by the TNBS enema method.The rats in the blank control group were given an equal amount of 0.9%sodium chloride solution.In the intervention phase,the blank control and model groups were given clean-grade rat food,while the high-FODMAP and low-FODMAP groups were given corresponding custom-made rat food,together with mesalazine treatment;while the blank control and model groups were given the same amount of 0.9%sodium chloride solution treatment.The intervention measures were given for 14 days.The general condition,disease activity index(DAI),gross morphological score of colon,histopathological score,interleukin 17(IL-17)and tumor necrosis factor(TNF-α)levels in serum and colon tissue were compared among groups.Results The DAI scores of rats in the model group,low FODMAP group and high FODMAP group were significantly higher than those in the blank control group(all P<0.05).Compared with the model group,the DAI scores in low FODMAP group at d7 and d14 were decreased significantly(P<0.05),while the DAI scores in high FODMAP group at 14 were significantly decreased(P<0.05).DAI score in high FODMAP group were significantly higher than that in low FODMAP group at d7 and d14(P<0.05).The gross morphological scores of the colon in the model group and the high FODMAP group were significantly higher than those in the blank control group(all P<0.05).Compared with the model group,the gross morphological scores of low FODMAP group and high FODMAP group were significantly lower(all P<0.05),while the gross morphological injury score in high FODMAP was significantly higher than that in low FODMAP group(P<0.05).Compared with the blank control group,the levels of TNF-αand IL-17 inflammatory factors in the colon

关 键 词：溃疡性结肠炎 低FODMAP饮食 美沙拉嗪 白细胞介素17 肿瘤坏死因子 

分 类 号：R574.62[医药卫生—消化系统] R-332[医药卫生—内科学]