miR1290在子宫内膜癌发生发展中的作用及其机制  

Effect of miR1290 on the growth of endometrial cancer and the related mechanism

作　　者：申晓畅 孙一卿 颜磊 赵兴波[1] Shen Xiaochang;Sun Yiqing;Yan Lei;Zhao Xingbo(Department of Gynecology,Shandong Provincial Hospital,Cheeloo College of Medicine,Shandong University,Jinan 250021,China;Department of Gynecology,Center for Reproductive Medicine,Cheeloo College of Medicine,Shandong University,Jinan 250021,China)

机构地区：[1]山东大学附属省立医院妇科,济南250021 [2]山东大学附属生殖医院妇科,济南250021

出　　处：《中华肿瘤杂志》2022年第2期130-138,共9页Chinese Journal of Oncology

基　　金：山东省自然科学基金项目(ZR2018MH207)。

摘　　要：目的探讨miR1290在子宫内膜癌组织中的表达情况及其与子宫内膜癌病理分级的关系, miR1290对子宫内膜癌细胞生物学特性的影响及其机制。方法收集2020年5月至2020年10月于山东大学附属省立医院手术切除的38例子宫内膜样腺癌组织、10例癌旁组织和23例正常子宫内膜组织, 采用逆转录聚合酶链反应检测其miR1290的表达。通过慢病毒转染敲低子宫内膜癌细胞KLE和Ishikawa中miR1290的表达, 细胞计数试剂盒8(CCK-8)法和集落形成实验检测细胞的增殖能力, 划痕实验和Transwell实验检测细胞的侵袭、迁移能力, Western blot法检测上皮-间充质转化(EMT)、磷脂酰肌醇3激酶(PI3K)/Akt和Wnt/β-catenin通路相关蛋白的表达。结果子宫内膜癌组织中miR1290的相对表达量为5.40±3.20, 为正常子宫内膜组织的1.55倍(P<0.01), 为癌旁组织的1.75倍(P<0.01)。17例增生期和6例分泌期子宫正常内膜组织中miR1290的相对表达量分别为3.00±1.08和4.97±0.58, 差异有统计学意义(P<0.01)。在KLE细胞和Ishikawa细胞中, 相对于Sh-NC组, Sh-miR1290组细胞的miR1290表达降低, CCK-8法检测的吸光度、集落形成实验检测的集落形成率升高, Transwell实验检测的穿膜细胞数、划痕实验检测的划痕愈合率降低(均P<0.05)。敲低miR1290, 可导致KLE细胞中EMT相关蛋白N-cadherin、Vimentin、Snail和Slug表达降低(均P<0.05), PI3K和P-Akt/Akt的表达降低(均P<0.05), Wnt和β-catenin的表达未见明显变化(均P>0.05);而Ishikawa细胞中EMT相关蛋白N-cadherin、Snail和Slug表达降低, E-cadherin表达升高(均P<0.05), Wnt和β-catenin的表达降低(均P<0.05), 而PI3K和P-Akt/Akt的表达未见明显变化(均P>0.05)。结论子宫内膜癌组织中miR1290的表达高于癌旁组织和正常子宫内膜组织。敲低miR1290的表达可以促进子宫内膜癌细胞增殖, 但抑制细胞的侵袭、迁移及EMT能力, 可能通过PI3K/Akt和Wnt/β-catenin通路发挥作用。Objective To explore the expression of miR1290 in endometrial cancer tissues and its relationship with the pathological grade,and to find out the effect of miR1290 on biological characteristics of endometrial cancer cells and its mechanism.Methods A total of 38 cases of endometrioid adenocarcinoma tissues,10 cases of adjacent tissues and 23 cases of normal endometrial tissues were collected in Provincial Hospital Affiliated to Shandong University from May 2020 to October 2020.The expression of miR1290 was detected by reverse transcription polymerase chain reaction(RT-PCR).The expressions of miR1290 in endometrial cancer cells including KLE and Ishikawa were knocked down by lentiviral transfection.Cell counting kit 8(CCK-8)test and colony formation test were used to detect cell proliferation ability,wound healing and Transwell test were used to detect cell invasion and migration ability,western blot was used to detect the expressions of epithelial-mesenchymal transition(EMT),phospholipids acylinositide 3-kinase(PI3K)/Akt and Wnt/β-catenin pathway related proteins.Results The relative expressions of miR1290 in endometrial cancer tissues were 5.40±3.20,which was 1.55 times of normal endometrial tissues(P<0.01)and 1.75 times of adjacent tissues(P<0.01).The relative expressions of miR1290 in 17 cases of endometrial tissues at proliferative stage and 6 cases of endometrial tissues at secretory stage were 3.00±1.08 and 4.97±0.58,respectively,and the difference was statistically significant(P<0.01).In KLE cells and Ishikawa cells,the expression of miR1290 in miR1290 knockdown(Sh-miR1290)group was decreased when compared with the negative control(Sh-NC)group.The absorbance value of Sh-miR1290 group detected by the CCK-8 method and the colony formation rate detected by the colony formation experiment were both increased,the number of cells penetrating the basement membrane in the Transwell experiment and the wound healing rate in the scratch experiment were decreased(P<0.05).In KLE cells,knockdown of miR1290 reduced th

关 键 词：子宫内膜肿瘤 miR1290 增殖 侵袭、迁移 EMT 信号通路 

分 类 号：R737.33[医药卫生—肿瘤]