复方丹参滴丸通过调控凋亡相关蛋白的表达抑制TNF-α诱导的人牙周膜成纤维细胞凋亡  

Compound danshen dripping pills inhibited TNF-αinduced apoptosis of human periodontal ligament cells by regulating the expression of apoptosis-related proteins

作　　者：江瑾 于春华 仇妮 王芳[3] 张明[3] JIANG Jin;YU Chunhua;QIU Ni;WANG Fang;ZHANG Ming(Qingdao Central Hospital,Qingdao 266000)

机构地区：[1]青岛市中心医院口腔科,266000 [2]青岛市中心医院药学部,266000 [3]青岛大学附属青岛市口腔医院种植科

出　　处：《现代口腔医学杂志》2021年第6期367-370,共4页Journal of Modern Stomatology

基　　金：青岛市医疗卫生重点学科建设项目;青岛市医疗卫生优秀人才培养项目;山东省医药卫生科技发展计划项目(2018WS365)。

摘　　要：目的探讨复方丹参滴丸(compound danshen dripping pills,CDDP)对肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)诱导的人牙周膜成纤维细胞(human periodontal ligament cells,hPLFCs)凋亡和凋亡相关蛋白的影响。方法体外培养hPLFCs,MTT法检测TNF-α对细胞增殖的影响,检测CDDP对于TNF-α诱导hPLFCs增殖的影响。Annexin V/PI检测CDDP对于TNF-α诱导hPLFCs凋亡率的变化。Western blot检测CDDP对于TNF-α诱导的hPLFCs凋亡相关蛋白p53、Bax及Bcl-2的影响。结果结果显示,与对照组比较,hPLFCs细胞经TNF-α处理后,细胞增殖显著下降,细胞凋亡率显著增加,p53和Bax表达水平明显升高,Bcl-2表达水平明显降低。与TNF-α组比较,hPLFCs细胞经剂量依赖性CDDP处理后,细胞增殖逐渐升高,细胞凋亡率逐渐下降,p53和Bax表达逐渐下降,Bcl-2表达逐渐升高。结论CDDP可以剂量依赖性降低TNF-α诱导的hPLFCs凋亡率及凋亡相关蛋白的表达水平,对抑制hPLFCs凋亡有着重要意义。Objective To investigate the effects of compound danshen dripping pills(CDDP)on tumor necrosis factor-α(TNF-α)induced apoptosis and apoptosis-related proteins in human periodontal ligament cells(hPLFCs).Methods hPLFCs were cultured in vitro,and the effect of TNF-αon cell proliferation was detected by MTT method.The effect of CDDP on hPLFCs proliferation which induced by TNF-αwas detected by MTT method.Annexin V/PI was used to detect the effect of CDDP on hPLFCs apoptosis rate which induced by TNF-α.Western blot was used to detect the effects of CDDP on TNF-α-induced hPLFCs apoptosis related proteins p53,Bax and Bcl-2.Results The results showed that compared with the control group,the proliferation of hPLFCs was significantly decreased,the apoptosis rate was significantly increased,the expression of p53 and Bax was gradually increased,and the expression of Bcl-2 was gradually decreased in the TNF-αgroup.Compared with the TNF-αgroup,hPLFCs cells treated with dose-dependent CDDP showed increased proliferation,decreased apoptosis rate,decreased the expression of p53 and Bax,and increased the expression of Bcl-2.Conclusion CDDP could reduce the apoptosis rate and the expression level of apoptosis-related proteins of hPLFCs which induced by TNF-αin a dose-dependent manner,which is of great significance to inhibit the apoptosis of hPLFCs.

关 键 词：复方丹参滴丸 TNF-Α 人牙周膜成纤维细胞 凋亡 

分 类 号：R781.4[医药卫生—口腔医学]