4 Gy X线照射通过上调MMP-2/9表达增强骨肉瘤细胞迁移能力  

4 Gy X-ray irradiation enhances the migration of osteosarcoma cells by up-regulating MMP-2/9

作　　者：吕小立 东肃河 刘冬书 王争明 王思念 马静 贾舒寒 李峰生 江其生 LÜ Xiao-li;DONG Su-he;LIU Dong-shu;WANG Zheng-ming;WANG Si-nian;MA Jing;JIA Shu-han;LI Feng-sheng;JIANG Qi-sheng(Postgraduate Training Base of PLA Rocket Force Characteristic Medical Center,Soochow University,Beijing 100088,China;PLA Rocket Force Characteristic Medical Center,Beijing 100088,China;Postgraduate Training Base of PLA Rocket Force Characteristic Medical Center,Jinzhou Medical University,Beijing 100088,China)

机构地区：[1]苏州大学医学部火箭军特色医学中心研究生培养基地,北京100088 [2]火箭军特色医学中心,北京100088 [3]锦州医科大学火箭军特色医学中心研究生培养基地,北京100088

出　　处：《军事医学》2021年第11期843-847,867,共6页Military Medical Sciences

基　　金：国家自然科学基金面上项目(31770914)。

摘　　要：目的研究4 Gy X线照射对骨肉瘤细胞迁移能力的影响及其机制。方法将143B细胞和U2OS细胞分为对照组和4 Gy照射组,分别于照射后12、24、36和48 h,采用Transwell法检测细胞的迁移能力,Western印迹检测细胞MMP-2和MMP-9蛋白表达水平;siRNA法干扰细胞MMP-2/MMP-9表达。结果与对照组相比,4 Gy X线照射后,143B细胞和U2OS细胞12和48 h的迁移能力未发生显著变化(P>0.05),24和36 h的迁移能力均显著增强(P<0.05);同时,两种细胞的MMP-2和MMP-9蛋白表达水平在12和48 h无显著变化(P>0.05),在24及36 h显著升高(P<0.05);干扰143B细胞和U2OS细胞中的MMP-2/MMP-9表达后,两种细胞的MMP-2/MMP-9蛋白表达水平及迁移能力均明显降低(P<0.05)。结论4 Gy X线照射可通过上调MMP-2/MMP-9表达水平增强143B细胞和U2OS细胞的迁移能力。Objective To investigate the effect of 4 Gy X-ray irradiation on migration of osteosarcoma cells and the mechanism.Methods 143 B cells and U2 OS cells were divided into control and 4 Gy irradiation groups.The cell migration ability was detected using Transwell method at 12,24,36 and 48 h after irradiation,and the cell MMP-2 and MMP-9 protein expression levels were detected by Western Blot at 12,24,36 and 48 h after irradiation.siRNA was used to interfere with cellular MMP-2/MMP-9 expressions.Results Compared with the control group,the migration ability of143 B cells and U2 OS cells did not change significantly(P>0.05)at 12 and 48 h,but was significantly enhanced(P<0.05)at 24 and 36 h after 4 Gy X-ray irradiation.Meanwhile,the expression levels of MMP-2 and MMP-9 proteins in either type of cell did not change significantly(P>0.05)at 12 and 48 h,and were significantly increased(P<0.05)at 24 and 36 h.At the same time,the MMP-2 and MMP-9 protein expression levels of both cells hardly changed at 12 and 48 h(P>0.05)but increased significantly at 24 and 36 h(P<0.05).After interference with MMP-2/MMP-9 expression in 143 B cells and U2 OS cells,the MMP-2/MMP-9 protein expression levels and migration ability of both types of cells decreased significantly(P<0.05).Conclusion 4 Gy X-ray irradiation can enhance the migration ability of 143 B cells and U2 OS cells by upregulating the expression level of MMP-2/MMP-9.

关 键 词：骨肉瘤 辐射 迁移 基质金属蛋白酶-2/-9 

分 类 号：R738.1[医药卫生—肿瘤]