基质固相分散和HPLC-IT-TOF/MS联用研究漆树木材中多酚成分  

Study on Polyphenols in Lacquer Tree by Matrix Solid-phase Dispersion and HPLC-IT-TOF/MS

作　　者：陈虹霞[1] 周昊[1,2] 陶冉[1] 齐志文 李文君[1] 王成章[1,2] CHEN Hongxia;ZHOU Hao;TAO Ran;QI Zhiwen;LI Wenjun;WANG Chengzhang(Institute of Chemical Industry of Forest Products,CAF,National Engineering Lab. for Biomass Chemical Utilization, Key Lab. of Chemical Engineering of Forest Products,National Forestry and Grassland Administration,Key Lab. of Biomass Energy and Material,Jiangsu Province,Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources,Nanjing 210042,China;Research Institute of Forestry New Technology,CAF,Beijing 100091,China)

机构地区：[1]中国林业科学研究院林产化学工业研究所,生物质化学利用国家工程实验室,国家林业和草原局林产化学工程重点实验室,江苏省生物质能源与材料重点实验室,江苏省林业资源高效加工利用协同创新中心,江苏南京210042 [2]中国林业科学研究院林业新技术研究所,北京100091

出　　处：《林产化学与工业》2022年第1期10-20,共11页Chemistry and Industry of Forest Products

基　　金：国家重点研发计划资助项目(2017YFD0601305);江苏省生物质能源与材料重点实验室开放基金资助项目(JSBEM-S-201908)。

摘　　要：采用基质固相分散(MSPD)技术进行漆树木中多酚化合物的提取,根据单因素试验得到优化工艺条件为:ZSM-22为吸附基质,吸附基质与样品的质量比为2∶1,洗脱溶剂为体积分数70%甲醇,洗脱溶剂用量8 mL,研磨时间2 min。在此条件下,漆树木多酚提取得率为(466±2.05)mg/g(以没食子酸计)。采用高效液相色谱-离子阱-飞行时间质谱(HPLC-IT/TOF-MS)联用技术,建立高效、准确鉴定漆树木中多酚化学成分的方法,根据质谱图和裂解规律信息,并结合文献,共鉴定10个化合物,包括3个没食子酸(GAC)类化合物和7个黄酮类化合物,分别是没食子酸、没食子酸甲酯、没食子酸乙酯、黄颜木素、花旗松素、3,4′,7-三羟基黄烷酮、异鼠李素、槲皮苷、漆黄素和硫黄菊素。利用基质固相分散和HPLC-IT/TOF-MS技术有利于漆树木中化合物的发现和鉴别,对漆树提取物的质量评价和后续分析具有重要的意义。Matrix solid-phase dispersion was proposed to extract polyphenols in Toxicodendron vernicifluum(Stokes)F.A.Barkl.(Shaanxi lacquer tree).The MSPD conditions to extract polyphenols established by the single factor test were ZSM-22 as the adsorbent,adsorbent/sample ratio of 2∶1(g∶g),grinding for 2 min,and use of 8 mL of 70%methanol as the elution solvent.Under these conditions,the polyphenols content of MSPD extraction was(466±2.05)mg/g(calculated by gallic acid).An efficient and accurate method for identification of polyphenols in Shaanxi lacquer tree was established by HPLC-IT-TOF/MS.According to mass spectrometry,fragmentation pathway combined with published reports,total of 10 components were identified,including 3 gallic acid derivatives and 7 flavonoids as follows:gallic acid,methyl gallate,ethyl gallate,fustin,taxifolin,garbanzol,isorhamnetin,quercitrin,fisetin,sulfuretin.It was beneficial to the discovery and identification of compounds in Shaanxi lacquer tree by matrix solid-phase dispersion coupled with HPLC-IT-TOF/MS,and it could be significance for the analysis and quantification of extracts from Shaanxi lacquer tree samples.

关 键 词：基质固相分散 漆树 多酚 高效液相色谱-离子阱-飞行时间质谱 

分 类 号：TQ351[化学工程]