miRNA-182、miRNA-375在结直肠癌患者外周血中的表达及与预后的关系  

Expression of miRNA-182 and miRNA-375 in peripheral blood of patients with colorectal cancer and their relationship with prognosis

作　　者：吕晓芳[1] 方立峰[1] 陈佩[1] LYU Xiaofang;FANG Lifeng;CHEN Pei(Department of Gastroenterology,Zhengzhou First People’s Hospital,Zhengzhou 450000,He’nan,China)

机构地区：[1]郑州市第一人民医院消化内科,郑州4500000

出　　处：《癌症进展》2022年第1期34-37,共4页Oncology Progress

基　　金：河南省医学科技攻关计划项目(LHGJ20200699)。

摘　　要：目的探究微小RNA-182(miRNA-182)、miRNA-375在结直肠癌患者外周血中的表达及与预后的关系。方法采用定量荧光聚合酶链反应(PCR)扩增技术对治疗前82例结直肠癌患者血浆miRNA-182、miRNA-375水平进行检测,并根据患者结直肠癌根治术后1年预后结果分为预后良好组和预后不佳组,收集并整理患者的一般资料,采用Logistic回归分析影响结直肠癌患者预后的相关因素,以受试者工作特征(ROC)曲线评估外周血miRNA-182、miRNA-375水平对患者预后的预测价值。结果82例结直肠癌患者中,预后不佳37例(预后不佳组),预后良好45例(预后良好组)。预后不佳组患者分化程度为低分化、肿瘤生长方式为浸润型所占比例及外周血miRNA-182、miRNA-375水平均高于预后良好组,差异均有统计学意义(P﹤0.05)。Logistic回归分析结果显示,肿瘤分化程度为低分化、肿瘤生长方式为浸润型、外周血miRNA-182及miRNA-375水平偏高均为结直肠癌患者预后不佳的危险因素(P﹤0.01)。ROC分析显示,外周血miRNA-182、miRNA-375单独及联合检测预测结直肠癌患者预后的曲线下面积(AUC)分别为0.80、0.82、0.88,外周血miRNA-182、miRNA-375单独检测对结直肠癌患者预后的预测效能低于二者联合。结论外周血miRNA-182、miRNA-375水平偏高的结直肠癌患者更易出现淋巴结转移等预后不佳情况,且miRNA-182、miRNA-375水平均对结直肠癌患者的预后具有一定的预测效能。Objective To explore the expression of microRNA-182(miRNA-182)and miRNA-375 in peripheral blood of patients with colorectal cancer and their relationship with prognosis.Method Plasma miRNA-182 and miRNA-375 of 82 patients with colorectal cancer before treatment were detected by quantitative fluorescent polymerase chain reaction(PCR)amplification technology,and the patients were divided into the good prognosis group and poor prognosis group according to the prognosis results within 1 year after radical resection of colorectal cancer.The general information of patients was collected and Logistic regression analysis was used to analyze the relevant factors affecting the prognosis of colorectal cancer patients.The receiver operating characteristic(ROC)curve was used to evaluate the predictive value of peripheral blood miRNA-182 and miRNA-375 on the clinical prognosis of patients.Result There were 37 cases of poor prognosis(poor prognosis group)and 45 cases of good prognosis(good prognosis group)in 82 patients with colorectal cancer.The proportion of poor differentiation,infiltrating tumor growth pattern and levels of peripheral blood miRNA-182 and miRNA-375 were higher than those in the good prognosis group(P<0.05).The results of Logistic regression showed that poorly differentiated,infiltrating tumor growth pattern,and high levels of miRNA-182 and miRNA-375 in peripheral blood were all risk factors for poor prognosis in patients with colorectal cancer(P<0.01).ROC analysis shows that the area under the curve(AUC)of single and combined miRNA-182 and miRNA-375 in predicting prognosis of patients with colorectal cancer was 0.80,0.82,and 0.88,respectively,with lower predictive power for the single detection than the combination detection(P<0.05).Conclusion Colorectal cancer patients with high levels of miRNA-182 and miRNA-375 in peripheral blood are more likely to have poor prognosis such as lymph node metastasis,and the levels of miRNA-182 and miRNA-375 have certain predictive power for the prognosis of patients with co

关 键 词：miRNA-182 miRNA-375 结直肠癌 外周血 预后 

分 类 号：R735.3[医药卫生—肿瘤]