白头翁皂苷A对肝癌细胞增殖、迁移、侵袭和放疗敏感性的影响及机制  

Effects of PSA on proliferation,migration,invasion,and radiosensitivity of liver cancer cells

作　　者：尹玉婷 黄炜 XI Yan YIN Yuting;HUANG Wei;XI Yan(Department of Pharmacy,Longhua Hospital Affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai 200032,China)

机构地区：[1]上海市中医药大学附属龙华医院药学部,上海200032 [2]不详

出　　处：《山东医药》2022年第7期39-42,共4页Shandong Medical Journal

摘　　要：目的 探讨白头翁皂苷A(PSA)对肝癌细胞增殖、迁移、侵袭和放疗敏感性的影响及机制。方法 体外培养HepG2细胞,取对数生长期的细胞分别用不同浓度(0、10、15、20 mg/L)PSA进行干预24 h,分别将0、20 mg/L的PSA干预的细胞记为对照组、PSA组。用MTT法检测两组细胞增殖能力,Transwell实验检测细胞迁移、侵袭能力,克隆形成实验、单击多靶模型检测肝癌细胞放疗敏感性,qRT-PCR检测细胞内SIRT1 mRNA表达,用Western blotting法检测细胞内SIRT蛋白表达。结果 随PSA浓度的增加,HepG2细胞活力、迁移细胞数目、侵袭细胞数目均逐渐降低(P均<0. 05)。经0、2、4、6、8 Gy X射线照射后,与对照组比较,PSA组细胞存活分数低(P均<0. 05);PSA组放射增敏比为1. 963。与对照组比较,PSA组SIRT1 mRNA、蛋白表达低(P均<0. 05)。结论 PSA可能通过下调SIRT1表达抑制肝癌细胞的增殖、迁移和侵袭,提高其放疗敏感性。Objective To investigate the effects and mechanism of Pulsatilla saponins A(PSA)on the proliferation,migration,invasion,and radiosensitivity of liver cancer cells.Methods Liver cancer cells HepG2 were cultured in vitro.HepG2 cells in the logarithmic growth phase were intervened with different concentrations of PSA(0,10,15 and 20 mg/L)for 24 h.Cells intervened with 0 and 20 mg/L PSA were taken as the control group and PSA group,respectively.MTT was used to detect cell viability in each group,Transwell was used to detect cell migration and invasion in each group,clone formation experiment and single-click multi-target model were used to detect radiotherapy sensitivity of liver cancer cells,qRT-PCR was used to detect the expression of SIRT1 mRNA,and the protein expression of SIRT1was detected by Western blotting.Results With the increased concentrations of PSA,the cell viability,the number of migration cells,and the number of invasion cells decreased(all P<0.05).Therefore,20 mg/L PSA was selected for subsequent experiments.After 0,2,4,6,and 8 Gy X-ray irradiation,the PSA group had a lower cell survival score compared with that of the control group(all P<0.05);the PSA group's sensitization enhancement ratio was 1.963.Compared with the control group,the expression levels of SIRT1 mRNA and protein in the PSA group were lower(both P<0.05).Conclusion PSA may inhibit the proliferation,migration,and invasion of liver cancer cells by down-regulating SIRT1,and then promote their radiosensitivity.

关 键 词：肝癌 白头翁皂苷A HEPG2细胞 细胞增殖 细胞迁移 细胞侵袭 放疗敏感性 

分 类 号：R735.7[医药卫生—肿瘤]