微小RNA-195通过靶向BIRC5调控肝癌Hep3B细胞凋亡实验研究  

Experimental study of miRNA-195 regulating apoptosis of hepatoma Hep3B cells by targeting BIRC5

作　　者：李玉龙[1] 宗伟[1] 常素娥 陈思攀[3] LI Yulong;ZONG Wei;CHANG Su’e;CHEN Sipan(Department of Gastroenterology,Shaanxi Provincial People’s Hospital,Xi’an 710068,China)

机构地区：[1]陕西省人民医院消化内科,陕西西安710068 [2]西安交通大学第二附属医院骨科,陕西西安710004 [3]陕西省人民医院介入放射科,陕西西安710068

出　　处：《陕西医学杂志》2022年第3期270-273,279,共5页Shaanxi Medical Journal

基　　金：国家自然科学基金资助项目(81601081);陕西省自然科学基础研究计划项目(2020JM-654)。

摘　　要：目的:探讨微小RNA-195(miR-195)对肝癌Hep3B细胞凋亡的影响,并探索其下游靶基因。方法:采用MTT实验、流式细胞仪检测miR-195对肝癌Hep3B细胞增殖及凋亡的影响。构建双荧光素酶报告基因质粒,采用双荧光素酶报告基因实验验证靶标分子。采用RNA干扰技术检测沉默BIRC5对肝癌Hep3B细胞增殖及凋亡的影响。结果:与miR-ctrl组相比,miR-195过表达可抑制肝癌Hep3B细胞增殖,促进其凋亡。双荧光素酶报告基因实验表明,miR-195可靶向作用BIRC5,沉默BIRC5可抑制肝癌Hep3B细胞增殖,促进其凋亡。结论:miR-195可通过靶向调控BIRC5抑制肝癌Hep3B细胞增殖,促进其凋亡。BIRC5可能是肝癌靶向治疗的重要靶标。Objective:To investigate the effect of microRNA-195(miR-195)on apoptosis of hepatoma Hep3B cells and explore its downstream target genes.Methods:MTT assay and flow cytometry were used to detect the effect of miR-195 on the proliferation and apoptosis of hepatoma Hep3B cells.Dual luciferase reporter gene vector was constructed,and double fluorescent reporter gene experiment was used to verify the targeting relationship between them.The effects of silencing BIRC5 on proliferation and apoptosis of hepatoma Hep3B cells were detected by RNA interference.Results:Compared with miR-ctrl group,miR-195 overexpression could inhibit the proliferation and promote the apoptosis of hepatoma Hep3B cells.Double fluorescent reporter gene experiment showed that miR-195 could target BIRC5,and silencing BIRC5 could inhibit the proliferation and promote apoptosis of hepatoma Hep3B cells.Conclusion:MiR-195 can inhibit the proliferation and promote the apoptosis of hepatoma Hep3B cells by targeting BIRC5.BIRC5 may be an important target for targeted therapy of liver cancer.

关 键 词：肝癌 微小RNA-195 靶基因 细胞增殖 细胞凋亡 BIRC5 

分 类 号：R735.7[医药卫生—肿瘤]