中性纤维素酶促进牛粪厌氧消化性能的研究  

Study on Promoting Anaerobic Digestion of Cow Manure by Adding Neutral Cellulase

作　　者：张成明 董保成[3,4] 李砚飞 刘晓玲 白耀博[1] 曹丹[1] 刘洁[1] 李十中 ZHANG Cheng-ming;DONG Bao-cheng;LI Yan-fei;LIU Xiao-ling;BAI Yao-bo;CAO Dan;LIU Jie;LI Shi-zhong(Xuzhou Biopharmaceutical and Waste Comprehensive Engineering Technology Research Center,Xuzhou Vocational College of Bioengineering, Xuzhou 221006, China;Institute of Nuclear and New Energy Technology,MOST-USDA Joint Research Center for Biofuels, Tsinghua University, Beijing 100084, China;Rural Energy and Environment Agency, Beijing 100125, China;School of Chemical Engineering and Technology, Tianjin University, Tianjin 300350, China;Qingxian New Energy Technology Promotion Center, Cangzhou 062650, China;Chinese Research Academy of Environmental Sciences, Beijing 100012, China)

机构地区：[1]徐州生物工程职业技术学院,徐州市生物制药与废弃物综合利用工程中心,江苏徐州221006 [2]清华大学,核能与新能源技术研究院,中美生物燃料联合研究中心,北京100084 [3]农业农村部农业生态与资源保护总站,北京100125 [4]天津大学化工学院,天津300350 [5]青县新能源技术推广中心,河北沧州062650 [6]中国环境科学研究院,北京100012

出　　处：《中国沼气》2022年第1期33-37,共5页China Biogas

基　　金：养殖粪污沼液回用工艺建立及关键抑制因子研究(青年人才)(KC18016)。

摘　　要：试验考察直接添加中性纤维素酶对牛粪沼气发酵性能的影响。向厌氧消化体系添加50,100,150,200 FPU·g^(-1)的中性纤维素酶,通过产气数据、发酵终点沼液性质来估计评估中性纤维素酶对牛粪沼气发酵性能的影响。中性纤维素酶可以促进牛粪的厌氧消化性能。当酶添加量为50 FPU·g^(-1)时,对牛粪产气没有影响;当酶添加量继续增加时,牛粪的累积甲烷产量开始增加,最高值为1085.6 mL·g^(-1),比对照高40%;当酶添加量超过100 FPU·g^(-1)时,单位酶对甲烷产量增加的贡献不再增加;当酶添加量超过150 FPU·g^(-1)时,牛粪产气高峰出现延迟的现象。高浓度的氨氮可能是造成产气延迟的原因。中性纤维素酶可以促进牛粪的沼气发酵性能,但当酶添加过多时,会引发沼气发酵的延迟,原因需要进一步分析。The effect of direct addition of neutral cellulase on biogas fermentation performance of cow manure was investigated.Adding 50,100,150,and 200 FPU·g^(-1)neutral cellulase to the anaerobic digestion system,the effect of neutral cellulase on cow manure biogas fermentation performance was evaluated by methane production and the properties of biogas slurry at the end of fermentation.Neutral cellulase could promote anaerobic digestion of cow manure.It had no effect on the methane production of cow manure when the enzyme dosage was 50 FPU·g^(-1).When the enzyme addition continued to increase,the cumulative methane production of cow manure began to increase,with the highest value of 1085.6 mL·g^(-1),which was 40%higher than that of the control.When the enzyme dosage was more than 100 FPU·g^(-1),the contribution of unit enzyme to methane production did not increase.When the enzyme dosage exceeded 150 FPU·g^(-1),the peak of biogas production was delayed.High concentration of ammonia nitrogen might be the reason for the delay of gas production.Neutral cellulase could promote the anaerobic digestion performance of cow manure.However,the methane production would be delayed when the enzyme dosage was too high,and the reason needs to be further analyzed.

关 键 词：牛粪 厌氧消化 氨氮 纤维素酶 

分 类 号：S216.4[农业科学—农业机械化工程] X713[农业科学—农业工程]