大鼠同种异体骨髓间充质干细胞联合Bio-Oss骨粉-bFGF复合物促进拔牙窝愈合的micro-CT研究  

Effect of rat allogeneic BMSCs-Bio-Oss-bFGF compound on tooth extraction healing:a micro-CT study

作　　者：江银华 商裕[1,2] 邹多宏 陈露[3] 费晨艳 梁凯 JIANG Yin-hua;SHANG Yu;ZOU Duo-hong;CHEN Lu;FEI Chen-yan;LIANG Kai(Lishui University.Lishui 323000,Zhengjiang Province;Lishui People's Hospital.Lishui 323000,Zhengjiang Province;Department of Oral Surgery,Shanghai Ninth People's Hospital,College of Stomatology,Shanghai Jiao Tong University School of Medicine.Shanghai 200011;Zhejiang Chinese Medical University.Hangzhou 310053,Zhejiang Province;Affiliated Stomatological Hospital of Nanchang University.Nanchang 330006,Jiangxi Province,China)

机构地区：[1]丽水学院,浙江丽水323000 [2]丽水市人民医院,浙江丽水323000 [3]上海交通大学医学院附属第九人民医院·口腔医学院口腔外科,上海200011 [4]浙江中医药大学,浙江杭州310053 [5]南昌大学附属口腔医院,江西南昌330006

出　　处：《上海口腔医学》2022年第1期38-43,共6页Shanghai Journal of Stomatology

基　　金：丽水市医学重点建设项目(2016zdxk08)。

摘　　要：目的:观察以Bio-Oss骨粉bFGF支架联合大鼠骨髓间充质干细胞(BMSCs)植入大鼠拔牙窝后在牙槽窝愈合过程中对牙槽骨微结构的影响。方法:取3周龄SD大鼠股骨全骨髓,自然贴壁分离BMSCs。选取36只6周龄SD大鼠,拔除上颌后牙,立即植入不同材料。按植入材料分为4组,即复合物组植入BMSCs联合Bio-Oss骨粉bFGF复合物、骨粉组植入Bio-Oss骨粉、干细胞组植入BMSCs和空白组直接对位缝合拔牙创。植入后4、12、24周,取上颌骨标本做micro-CT扫描。采用GraphPad Prism 6.0软件包对数据进行双因素方差分析。结果:植入第4周,骨密度(BMD)、骨小梁分离度(Tb.Sp)、骨小梁厚度(Tb.Th)、各向异性值(DA)及骨小梁数(Tb.N),复合物组与骨粉组、干细胞组与空白对照组均无显著差异(P>0.05)。复合物组BMD在植入第12周显著高于骨粉组、干细胞组和空白对照组(P<0.05);植入第24周显著高于骨粉组和空白对照组(P<0.05)。复合物组Tb.Th在第12周和第24周显著高于骨粉组(P<0.05)。第4、12、24周,复合物组与骨粉组、干细胞组及空白对照组DA均无显著差异(P>0.05)。复合物组Tb.Sp在植入后第24周显著小于骨粉组、干细胞组和空白对照组,复合物组Tb.N在植入后第24周显著高于干细胞组和空白对照组(P<0.05)。结论:大鼠同种异体BMSCs联合Bio-Oss骨粉-bFGF复合物对大鼠拔牙窝的愈合有促进作用。PRUPOSE:To investigate the effect of a compound of BMSCs-Bio-Oss-bFGF on microstructure of extraction sockets in rats.METHODS:Bone mesenchymal stem cells(BMSCs)were isolated from bone marrow of 3-week SD rats by adherent method.Maxillary posterior teeth of 366-week SD rats were extracted and materials were implanted into sockets according to grouping.The rats were divided into 4 groups:compound group with implanting BMSCs-Bio-Oss-bFGF compound,powder group with implanting Bio-Oss,BMSCs group with implanting BMSCs,and control group without implanting any materials.The sockets were scanned by micro-CT 4 weeks,12 weeks and 24 weeks after implantation.Two-way ANOVA was used to assess whether there was significant difference between groups with GraphPad Prism 6.0 software package.RESULTS:There was no significant difference among groups in bone mineral density(BMD),trabecular separation(Tb.Sp),trabecular thickness(Tb.Th),degree of anisotropy(DA),and trabecular number(Tb.N)4 weeks after implantation.By 12 weeks,BMD of compound group was significantly greater than those of BMSCs group,powder group and control group(P<0.05),and significantly greater than those of powder group and control group at 24 weeks(P<0.05).Tb.Th of compound group was significantly greater than that of BMSCs group at 12 and 24 weeks(P<0.05).DA had no significant difference among groups at 4,12,and 24 weeks(P>0.05).Tb.Sp of compound group was significantly smaller than those of powder group,BMSCs group and control group at 24 weeks(P<0.05).Tb.N of compound group was significantly greater than those of BMSCs group and control group(P<0.05).CONCLUSIONS:The compound of rat allogeneic BMSCs-Bio-Oss-bFGF improves socket healing.

关 键 词：骨髓间充质干细胞 BIO-OSS骨粉 BFGF 拔牙窝 MICRO-CT 

分 类 号：R782.1[医药卫生—口腔医学]