复合酶法提取蓝莓皮渣中白藜芦醇的工艺及其分离提纯  

Extraction resveratrol process and fractionation from blueberry peel residue using mulriple enzymes

作　　者：张华[1] 仇宏图 高青山[1] 王福超 ZHANG Hua;QIU Hongtu;GAO Qingshan;WANG Fuchao(School of Agronomy,Yanbian University,Yanji 133002;Xilingol Vocational College,Xilinhaote 026000)

机构地区：[1]延边大学农学院,延吉133002 [2]锡林郭勒职业学院,锡林浩特026000

出　　处：《安徽农业大学学报》2021年第6期1027-1034,共8页Journal of Anhui Agricultural University

基　　金：高等学校学科创新引智计划111项目(D20034)资助。

摘　　要：为提高蓝莓皮渣这一副产品的利用率,通过冷冻干燥技术,制得蓝莓皮渣粉末,通过单因素试验确定复合酶比例、酶解温度、酶解时间、pH值4个因素对蓝莓皮渣中白藜芦醇的含量的影响,采用响应面优化得出最优提取条件,并进行了LC-18SPE分离提纯。结果表明:液相色谱-质谱法(LC-MS)结果显示蓝莓皮渣中大多为酯类物质和醛、酮类物质;白藜芦醇最优提取条件为料液(乙醇)比1:20(g·mL^(-1)),复合酶比例(纤维素酶:果胶酶)1:5,酶解温度54.62℃,酶解时间1 h,pH值5.38,白藜芦醇提取含量最高为(236±0.424)mg·kg^(-1)。利用乙酸乙酯分提含量最高达到76.61%。Blueberry peel power(BPP)was obtained by freeze-drying technology to improve the utilization rate of blueberry peel as a by-product.The contents of resveratrol from blueberry powder were determined by single experiments on the ratio of complex enzymes,enzymolysis temperature,enzymolysis time and pH values to explore the optimal conditions,and LC-18 SPE was used to separate and purify BPP,which was mainly composed of esters,aldehydes and ketones.The optimal conditions for BPP extraction were based on the ratio of solid(BBP)to liquid(ethanol)of 1:20(g·mL^(-1)),the ratio of compound enzyme(cellulase:pectinase)of 1:5,enzymolysis at 54.62℃for 1 h with pH value of 5.38,and the extraction rate of resveratrol content was(236±0.424)mg·kg^(-1).The fractionation content with ethyl acetate was up to 76.61%.

关 键 词：蓝莓皮渣 复合酶法 白藜芦醇 分离提纯 

分 类 号：S58[农业科学—作物学]