小花清风藤醇提物的化学成分及体外抗氧化活性研究  

Study onchemicalconstituentsand in vitro antioxidant activityofethanolextractof Sabia parviflora

作　　者：周永强 赵春丽 殷鑫 周涛 韩伟 张永萍 ZHOU Yongqiang;ZHAO Chunli;YIN Xin;ZHOU Tao;HAN Wei;ZHANG Yongping(College of Pharmacy,Guizhou University of Traditional Chinese Medicine,Guiyang 550025,China)

机构地区：[1]贵州中医药大学药学院,贵阳550025

出　　处：《中国药房》2022年第5期530-534,共5页China Pharmacy

基　　金：国家自然科学基金-贵州喀斯特中心项目(No.U1812403);贵州省科技计划项目(No.黔科合基础-ZK〔2021〕一般518);贵州省教育厅青年科技人才成长项目(No.黔教合KY字〔2018〕206);贵州省中医药管理局中医药、民族医药科学技术研究课题(No.QZYY-2020-040)。

摘　　要：目的分离、鉴定小花清风藤70%乙醇提取物的化学成分,并初步评价其体外抗氧化活性。方法采用硅胶、ODS反相硅胶、Sephadex-LH20柱色谱法及制备型高效液相色谱法等对小花清风藤70%乙醇提取物中化学成分进行分离和纯化,并结合核磁共振氢谱(^(1)H-NMR)、核磁共振碳谱(^(13)C-NMR)及电喷雾-质谱(ESI-MS)等波谱方法鉴定所分离出的化合物结构。分别采用2,2-联苯基-1-苦基肼基自由基(DPPH·)、2,2′-联氮基双(3-乙基苯并噻唑啉-6-磺酸)二铵盐自由基(ABST+)和羟基自由基(OH·)清除法考察各化合物的体外抗氧化活性。结果从小花清风藤70%乙醇提取物中共分离得到9个化合物,分别鉴定为芦丁(1)、邻苯二甲酸二异壬酯(2)、邻苯二甲酸二丁酯(3)、吐叶醇(4)、杜鹃醇(5)、槲皮素-3-O-龙胆双糖苷(6)、水仙苷(7)、山柰酚-3-O-芸香糖苷(8)、bonaroside(9)。体外抗氧化结果显示,化合物1~9均表现出一定的体外抗氧化活性,其中化合物1、6、7、8对DPPH·、ABST^(+)、OH·的半数清除浓度均低于70μg/mL。结论首次从清风藤属植物中分离得到吐叶醇、杜鹃醇、bonaroside;芦丁、槲皮素-3-O-龙胆双糖苷、水仙苷、山柰酚-3-O-芸香糖苷表现出较好的体外抗氧化活性。OBJECTIVE To separate and identify the chemical constituents in 70%ethanol extract of Sabia parviflora,and to preliminarily evaluate their in vitro antioxidant activity.METHODS The chemical constituents were separated and purified by silica gel,ODS reversed-phase silica gel,Sephadex-LH20 column and preparative high performance liquid chromatography.The structures of the isolated compounds were identified by ^(1)H-NMR,^(13)C-NMR and ESI-MS.The in vitro antioxidant activities of the compounds were investigated by 2,2-diphenyl-1-picrylhydrazyl radical(DPPH·),2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonate)diammonium radical(ABST+)and hydroxyl radical(OH·).RESULTS A total of 9 compounds were isolated from the 70%ethanol extracts of S.parviflora.They were identified as rutin(1),diiononyl phthalate(2),dibutyl phthalate(3),vomifoliol(4),rhododendrol(5),quercetin-3-O-gentiobioside(6),narcissoside(7),kaempferol-3-O-rutinoside(8)and bonaroside(9).The in vitro antioxidant results showed that compound 1-9 showed certain in vitro antioxidant activity,and the half scavenging concentrations of compound 1,6,7 and 8 to DPPH·,ABST^(+),OH·were lower than 70μg/mL.CONCLUSIONS Vomifoliol,rhododendrol and bonaroside are isolated from S.parviflora for the first time,and rutin,quercetin-3-O-gentiobioside,narcissoside and kaempferol-3-O-rutinoside show good in vitro antioxidant activity.

关 键 词：小花清风藤 化学成分 70%乙醇提取物 抗氧化活性 

分 类 号：R917[医药卫生—药物分析学] R285[医药卫生—药学]