托盘根中总多酚提取工艺及抗氧化活性研究  

Extraction and Antioxidant Activity of Total Polyphenols from Rubus Crataegifolius Bunge Roots

作　　者：赵楠 张春楠 柴军红[1] 何婷婷[1] ZHAO Nan;ZHANG Chun-nan;CHAI Jun-hong;HE Ting-ting(College of Life Science and Technology,Mudanjiang Normal University,Mudanjiang 157011,China)

机构地区：[1]牡丹江师范学院生命科学与技术学院,黑龙江牡丹江157011

出　　处：《化学试剂》2022年第3期364-368,共5页Chemical Reagents

基　　金：黑龙江省教育厅项目(135MSYYB004);牡丹江师范学院科研项目(QN2019004)。

摘　　要：以悬钩子属植物托盘根为研究对象,采用超声辅助乙醇提取托盘根中总多酚。通过对乙醇浓度、料液比、超声时间、超声温度4种影响因素进行考察,在单因素实验结果基础上,采用正交试验优化其最佳提取工艺,并对托盘根总多酚的抗氧化能力进行评价。结果表明,托盘根活性成分的最佳提取工艺为乙醇浓度60%、超声温度80℃、超声时间50 min、料液比1∶40 g/mL。在此条件下托盘根总多酚提取率为26.16%。托盘根总多酚清除DPPH自由基及羟基自由基的IC_(50)值分别为0.0069和0.346 mg/mL,表明托盘根总多酚具有一定的抗氧化能力。To investigate the total polyphenols from Rubus crataegifolius Bunge roots,ultrasonic-assisted ethanol extraction was used.By investigating four influencing factors(including ethanol concentration,ultrasonic temperature and time,and solid-liquid ratio),and using orthogonal experimental design on the basis of single factor experiments,the extraction was optimized,and the antioxidant activity was evaluated.The results suggested the following optimum conditions:ethanol concentration 60%,solid-liquid ratio 1∶40 g/mL,ultrasonic 50 min at 80℃.Under this condition,the extraction rate of total polyphenols reached 26.16%.The IC_(50) of total polyphenols from Rubus crataegifolius Bunge against DPPH and hydroxyl anion free radicals were 0.0069 and 0.346 mg/mL,respectively,which illustrated good antioxidant activity of the total polyphenols.

关 键 词：托盘 总多酚 超声提取 正交设计 抗氧化 

分 类 号：R284.2[医药卫生—中药学]