黑花生衣色素的提取及调节C2C12肌管细胞葡萄糖消耗的作用研究  

Extraction of black peanut skin pigment and its regulation of glucose consumption in C2C12 myotubes

作　　者：赵镇雷 徐小刚 高灿 张晓烨 常秀莲[2] 毛根祥[1] ZHAO Zhenlei;XU Xiaogang;GAO Can;ZHANG Xiaoye;CHANG Xiulian;MAO Genxiang(Zhejiang Provincial Key Lab of Geriatrics&Geriatrics Institute of Zhejiang Province,Department of Geriatrics,Zhejiang Hospital,Hangzhou 310013;School of Life Sciences,Yantai University,Yantai 264005)

机构地区：[1]浙江医院浙江省老年医学研究所浙江省老年医学重点实验室,杭州310013 [2]烟台大学生命科学学院,烟台264005

出　　处：《中国食品添加剂》2022年第2期60-69,共10页China Food Additives

基　　金：国家级星火计划项目资助(2014GA741008);国家自然科学基金(31702144)。

摘　　要：从花生加工中的副产物黑花生衣中提取纯化黑花生衣色素(BPSP),并初步探究其相关细胞生物学活性。首先,采用紫外可见(UV-vis)全光谱扫描及分光光度法对BPSP的检测条件、对温度和pH稳定性以及最佳提取条件进行了探究,发现BPSP在pH2~3、520nm可见波长时有最佳特征吸收峰,其在水溶液中比在乙醇溶液中更为稳定,BPSP水溶液在温度不高于50℃、pH为2.0的条件下稳定性最好;此外,BPSP的最佳提取条件为:70℃、pH2.0的酸性水溶液浸提15min,此条件下BPSP的提取率为7.95mg/g干黑花生衣重。进一步采用乙酸乙酯(EtOAc)萃取结合大孔树脂柱层析的方法对BPSP花色苷进行纯化,BPSP总花色苷纯度从粗提液的5.2%提高至25.4%,提高了近5倍。最后,选用纯化后的BPSP干预分化成熟的C2C12肌管细胞,发现5μg/mL剂量的BPSP能够显著增加C2C12肌管细胞对葡萄糖的消耗量(P<0.05),并且在1~5μg/mL的范围内呈现出剂量依赖关系。Black peanut skin pigment(PBSP)was extracted and purified from the black peanut skins which were regarded as a by-product of the peanut industry,and its related cell biological activities were preliminarily studied.First,the detection conditions,temperature and pH stability and optimum extraction conditions of BPSP were explored by UV-vis full-spectrum scanning technique and spectrophotometry.Results showed that BPSP has optimal characteristic absorption peak at pH2~3 and 520nm visible wavelength,and it is more stable in aqueous solution than in ethanol solution;also,BPSP aqueous showed the best stability when the temperature is not higher than 50℃at pH2.0;in addition,the optimal extraction conditions of BPSP were as follows:70℃,pH2.0 acidic aqueous solution extract for 15min;under this condition,the yield of BPSP was 7.95mg/g dry black peanut coat weigh.Further,BPSP anthocyanins were purified by ethyl acetate(EtOAc)partition combined with macroporous resin column chromatography technique,and the purity of total anthocyanins was increased nearly by 5-folds,from 5.2%(raw extraction)to 25.4%(after purification).Finally,purified BPSP was used to intervene the differentiated mature C2C12 myotubes.It was found that BPSP at the dose of 5μg/mL could significantly increase the glucose consumption of C2C12 myotube cells(P<0.05),moreover,within the concentration range of 1~5μg/mL,this promoting effect was dose-dependent.

关 键 词：黑花生衣 花色苷 天然色素 提取纯化 葡萄糖消耗 C2C12肌管细胞 

分 类 号：TS201.4[轻工技术与工程—食品科学]