反相离子对色谱法测定保健食品中硫酸软骨素和盐酸氨基葡萄糖  

Determination of chondroitin sulfate and glucosamine hydrochloride in healthy food by reversed phase ion pair chromatography

作　　者：冯雪萍 朱银宏 程倩 李春阳 陈昭 张海波 FENG Xueping;ZHU Yinhong;CHENG Qian;LI Chunyang;CHEN Zhao;ZHANG Haibo(Hubei Provincial Key Laboratory of Yeast Function,Yichang 443003;Angel Nutritech Co.,Ltd.,Yichang 443003)

机构地区：[1]酵母功能湖北省重点实验室,宜昌443003 [2]安琪纽特股份有限公司,宜昌443003

出　　处：《中国食品添加剂》2022年第2期186-193,共8页China Food Additives

摘　　要：建立检测保健食品中硫酸软骨素和盐酸氨基葡萄糖含量的反相离子对色谱分析方法。称取样品,以乙腈/水(5/95,v/v)混合溶液进行超声提取,稀释定容后,滤过。采用高效液相色谱法进行测定,以Agilent Zorbax SB-Aq(4.6×250mm,5μm)色谱柱作为分析柱,柱温30℃,流速0.8mL/min,流动相为乙腈/10mmol/L戊烷磺酸钠溶液(5/95,v/v),等度洗脱,以外标法定量。硫酸软骨素和盐酸氨基葡萄糖分别在0.1~0.75mg/mL和0.2~1.5mg/mL的浓度范围内呈良好线性关系,相关系数R^(2)分别为0.995和0.998;硫酸软骨素加标回收率在101.2%~105.4%之间,精密度RSD为0.3%;盐酸氨基葡萄糖加标回收率在102.1%~106.0%之间,精密度RSD为0.3%。此方法操作简单,准确性好,重复性好,适用于保健食品中硫酸软骨素和盐酸氨基葡萄糖的含量测定。To establish a method for the determination of chondroitin sulfate and glucosamine hydrochloride in dietary supplements by reversed phase ion pair chromatography.Chondroitin sulfate and glucosamine hydrochloride in samples were extracted with acetonitrile/water(5/95,v/v)by ultrasound,and diluted to a fixed volume,then passed through the membrane and detected by high performance liquid chromatography.The separation was conducted on a Agilent Zorbax SB-Aq chromatographic column(4.6×250mm,5μm),and the column temperature was set at 30℃.The flow rate was 0.8mL/min with a mobile phase of acetonitrile/10mM pentane solution of sodium(5/95,v/v)with a isocratic elution system.The chondroitin sulfate and glucosamine hydrochloride was quantified with external standard method.Chondroitin sulfate had a good linear relationship in the concentration range of 0.1~0.75mg/mL,with R^(2) of 0.995.Glucosamine hydrochloride had a good linear relationship in the concentration range of 0.2~1.5mg/mL,with R^(2) of 0.998.The recoveries of chondroitin sulfate from 101.2%to 105.4%,and in the precision experiment,the relative standard deviation is 0.3%.The recoveries of glucosamine hydrochloride from 102.1%to 106.0%,and in the precision experiment,the relative standard deviation is 0.3%.The method is simple,accurate and reproducible,and is suitable for the determination of chondroitin sulfate and glucosamine hydrochloride in dietary supplements.

关 键 词：硫酸软骨素 盐酸氨基葡萄糖 反相离子对色谱法(RPIC) 保健食品 检测 

分 类 号：O657.7[理学—分析化学] TS202.3[理学—化学]