宜昌地区结核分枝杆菌北京基因型菌株的鉴定及VNTR分型  

Identification and VNTR typing of Beijing genotype strains of Mycobacterium tuberculosis in Yichang area

作　　者：李丹 胡晓红[1,2] 徐新娟 杜德兵 吴文艳 王德成 庄倩 陈志杰[1,2] 金柱 LI Dan;HU Xiao-hong;XU Xin-juan;DU De-bing;WU Wen-yan;WANG De-cheng;ZHUANG Qian;CHEN Zhi-jie;JIN Zhu(Department of Pulmonary Di-seases,The Third People’s Hospital of Yichang,Yichang 443003,China;Institute of Infection and Inflammation,China Three Gorges University,Yichang 443002,China;Medical College of China Three Gorges University,Yichang 443002,China)

机构地区：[1]宜昌市第三人民医院肺病科,湖北宜昌443003 [2]三峡大学感染与炎症损伤研究所,湖北宜昌443002 [3]三峡大学医学院,湖北宜昌443002

出　　处：《中国感染控制杂志》2022年第3期232-238,共7页Chinese Journal of Infection Control

基　　金：湖北省卫生与健康委员会科研项目(WJ2019F080)。

摘　　要：目的研究宜昌地区结核分枝杆菌北京基因型菌株的多态性和流行特征,揭示该地区结核病的分子流行病学特征,为结核病防治提供科学依据。方法选择2018年1月—2019年12月具有完整病例信息的结核分枝杆菌298株,进行人型结核分枝杆菌的鉴定,用扩增RD105缺失片段的多重PCR(DTM-PCR)鉴定北京基因型菌株;应用优化的9位点数目可变串联重复序列技术(VNTR)分析北京基因型菌株的多态性。结果298株结核分枝杆菌中,260株为人型结核分枝杆菌,DTM-PCR鉴定发现北京基因型菌株140株(53.85%),非北京基因型菌株120株(46.15%)。北京基因型菌株耐药率(32.14%)高于非北京基因型菌株耐药率(10.83%),差异有统计学意义(P<0.05)。VNTR-9位点对北京基因型菌株的分辨率为0.9985,成簇率为12.15%。结论北京基因型菌株在宜昌地区呈较高流行趋势,且北京基因型菌株耐药率更高。VNTR-9位点基因分型方法用于该地区结核分枝杆菌北京基因型菌株的鉴别,能准确反映宜昌地区结核分枝杆菌的分子流行病学特征。Objective To study the polymorphism and epidemic characteristics of the Beijing genotype strains of Mycobacterium tuberculosis(Mtb)in Yichang area,reveal the molecular epidemiological characteristics of tuberculosis in this area,and provide scientific evidence for the prevention and treatment of tuberculosis.Methods 298 strains of Mtb with complete case information from January 2018 to December 2019 were selected for the identification of human Mtb,Beijing genotype strains were identified by multiplex polymerase chain reaction(DTM-PCR)which amplifying RD105 deletion fragment;polymorphism of Beijing genotype strains was analyzed by optimized 9-locus variable-number tandem repeat technique.Results Among 298 strains of Mtb,260 strains were human Mtb,DTM-PCR identification found that 140 strains(53.85%)were Beijing genotype and 120 strains(46.15%)were non-Beijing genotype.Drug resistance rate of Beijing genotype strains was higher than that of non-Beijing genotype strains(32.14%vs 10.83%,P<0.05).The resolution of VNTR-9 locus to Beijing genotype strains was 0.9985 and the clustering rate was 12.15%.Conclusion Beijing genotype strains showed a high prevalence trend in Yichang area,drug resistance rate of Beijing genotype strains is higher.VNTR-9 genotyping method used to identify Beijing genotype strains of Mtb in this area can accurately reflect the molecular epidemiological characteristics of Mtb in Yichang.

关 键 词：结核分枝杆菌 北京基因型 可变串联重复序列技术 分子流行病学 

分 类 号：R181.32[医药卫生—流行病学]