白藜芦醇促进人前列腺癌PC-3细胞凋亡机制的研究  

A study on the mechanism of resveratrol promoting apoptosis of human prostate cancer PC-3 cells

作　　者：李家萱 陈美霓[1] 郭巍 LI Jia-xuan;CHEN Mei-ni;GUO Wei(Medical School of Yan′an University, Shanxi 716000, China)

机构地区：[1]延安大学医学院,陕西716000 [2]延安市中医医院泌尿外科,陕西716000

出　　处：《中国临床新医学》2022年第2期124-128,共5页CHINESE JOURNAL OF NEW CLINICAL MEDICINE

基　　金：延安市科技计划项目(编号:SL2019ZCFY-004);延安大学校级青年项目(编号:YDY2020-18);延安大学省级大学生创新项目(编号:S202110719097)。

摘　　要：目的观察白藜芦醇(Res)对人前列腺癌PC-3细胞增殖、凋亡的影响,并探讨其机制。方法以PC-3细胞作为实验对象,并根据干预方式不同分为A组(12.50μmol/L Res)、B组(25.00μmol/L Res)、C组(50.00μmol/L Res)、D组(100.00μmol/L Res)和对照组(等体积完全培养基)。应用噻唑蓝(MTT)比色法检测各组PC-3细胞的增殖情况。应用碘化丙啶(PI)荧光染色观察各组PC-3细胞核变化情况。应用流式细胞仪检测各组PC-3细胞凋亡情况。通过实时荧光定量聚合酶链式反应(RT-qPCR)检测Caspase-3、Caspase-8、Caspase-9、Bax和Bcl-2的mRNA表达量。结果MTT比色法检测结果显示,以对照组作为参照,各实验组PC-3细胞生长抑制率均随干预时间的延长而升高,且B组、C组和D组在干预48 h后PC-3细胞增殖抑制作用明显。PI荧光染色结果显示,经Res干预后PC-3细胞出现凋亡形态,且随着Res干预浓度的上升,凋亡小体数量增加。流式细胞仪检测结果显示,A组、B组、C组和D组的PC-3细胞凋亡率分别为(1.35±0.65)%、(5.17±1.18)%、(10.74±2.24)%和(21.16±3.13)%,对照组为(0.77±0.11)%,差异有统计学意义(F=64.332,P=0.000)。RT-qPCR结果显示,与对照组比较,C组、D组Caspase-3、Caspase-9、Caspase-8、Bax mRNA相对表达量显著升高(P<0.05),Bcl-2 mRNA相对表达量显著降低(P<0.05)。结论Res具有抑制人前列腺癌PC-3细胞增殖和促进其凋亡的作用,这可能与调控Caspase-3/Bax/Bcl-2凋亡信号通路有关。Objective To observe the effect of resveratrol(Res)on the proliferation and apoptosis of human prostate cancer PC-3 cells,and to explore its mechanism.Methods The PC-3 cells were used as the experimental subjects and were divided into group A(12.50μmol/L Res),group B(25.00μmol/L Res),group C(50.00μmol/L Res),group D(100.00μmol/L Res)and control group(equal volume of complete medium)according to different intervention methods.The methyl thiazolyl tetrazolium(MTT)colorimetric method was used to detect the proliferation of the PC-3 cells in each group.Propidium iodide(PI)fluorescence staining was used to observe the changes of the PC-3 cell nuclei in each group.Flow cytometry was used to detect the apoptosis of the PC-3 cells in each group.Real-time quantitative polymerase chain reaction(RT-qPCR)was used to detect the mRNA expressions of Caspase-3,Caspase-8,Caspase-9,Bax and Bcl-2.Results The results of the MTT colorimetric method showed that,with the control group as a reference,the growth inhibition rate of the PC-3 cells in each experimental group increased with the extension of the intervention time,and the group B,the group C and the group D showed obvious PC-3 cell growth inhibitory effect after 48-hour intervention.The results of PI fluorescence staining showed that apoptotic morphology appeared in the PC-3 cells after the intervention of Res,and the number of apoptotic bodies increased with the increase of the concentration of Res intervention.The results of flow cytometry showed that the apoptotic rates of the PC-3 cells in the group A,the group B,the group C and the group D were(1.35±0.65)%,(5.17±1.18)%,(10.74±2.24)%and(21.16±3.13)%,respectively,and the apoptotic rate of the PC-3 cells in the control group was(0.77±0.11)%,and the differences were statistically significant(F=64.332,P=0.000).The results of RT-qPCR showed that compared with those in the control group,the relative expressions of Caspase-3,Caspase-9,Caspase-8 and Bax mRNA in the group C and the group D were significantly increased(P<0.

关 键 词：白藜芦醇 PC-3细胞 增殖抑制 凋亡 

分 类 号：R737.25[医药卫生—肿瘤]